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海岸环境中两类软质微塑料表面生物膜DNA的提取方法比较与优化

文献类型：期刊论文

作者陈涛; 涂晨; 郭鹏鹏; 周倩; 骆永明
刊名海洋学报
出版日期2018-12-15
卷号40期号:12页码:112-119
关键词微塑料 生物膜 DNA 聚乙烯薄膜(PE) 发泡类聚苯乙烯(EPS)
ISSN号0253-4193
DOI10.3969/j.issn.0253-4193.2018.12.011
其他题名Comparison and optimization of biofilm DNA extraction from two types of soft microplastics in the coastal environment
产权排序(1)中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室 ; (2)中国科学院大学 ; (3)中国科学院南京土壤研究所中国科学院土壤环境与污染修复重点实验室
英文摘要Microplastics could serve as carriers for heavy metals, organic pollutants and pathogens in the coastal and marine environment due to their small sizes and large specific surface area. It has been reported that the microbial colonizers attaching on the surface of microplasti cs mainly assemblaged in the form of biofilms. In this study, two types of soft microplastics, expanded polystyrene (EPS) and polyethylene films (PE), were selected as tested microplastics that were commonly found in the coastal environment in Shandong province. Two DNA extraction kits (MP FastDNA® and MOBIO PowerSoil®) were used to compare their DNA extraction efficiency from different microplastic biofilms. Moreover, impacts from different particle sizes and quantities on DNA extraction efficiency of microplastic-associated biofilms were also evaluated. The results showed that concentrations of biofilm DNA extracted by FastDNA® kit were significantly higher than by MOBIO PowerSoil®kit (1.0-12.5 times). DNA concentrations from PE biofilm were about 1.3-4.4 times higher than that from EPS extracted by FastDNA®. When the quantities of microplastics were no more than 20, DNA concentrations from small sizes (1-3 mm) EPS were significantly lower than that from large sizes (3-5 mm), while it was totally in opposition for PE. With the increasing of microplastics quantities, DNA concentrations were significantly increased in both two sizes of EPS. But for small sizes (1-3 mm) of PE, DNA concentration showed an increasing initially and decreasing afterward trend with the increasing of PE quantities. However, for large sizes (3-5 mm) of PE, DNA concentrations were decreasing with the increasing of PE quantities. Different impacts of microplastic sizes and quantities on DNA concentrations could be attributed to the types and physicochemical properties of microplastics. Results from this study will be helpful for providing methodological support for the research of microbial community composition and diversity of microplastic-associated biofilms in the coastal and marine environment.
资助项目Key Research Project of Frontier Science of Chinese Academy of Sciences[QYZDJ-SSW-DQC015] ; National Key R D projects[2016YFC1402202] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XDA11020305]
语种中文
CSCD记录号CSCD:6403199
资助机构中国科学院前沿科学重点研究项目#QYZDJ-SSW-DQC015 ; 科技部国家重点研发计划项目课题#2016YFC1402202 ; 中国科学院战略性先导科技专项子课题#XDA11020305
源URL[http://ir.yic.ac.cn/handle/133337/24317]  
专题烟台海岸带研究所_近岸生态与环境实验室
烟台海岸带研究所_污染过程与控制实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.中国科学院大学;
2.中国科学院南京土壤研究所中国科学院土壤环境与污染修复重点实验室
3.中国科学院烟台海岸带研究所中国科学院海岸带环境过程与生态修复重点实验室;
推荐引用方式
GB/T 7714		 陈涛,涂晨,郭鹏鹏,等. 海岸环境中两类软质微塑料表面生物膜DNA的提取方法比较与优化[J]. 海洋学报,2018,40(12):112-119.
APA 陈涛,涂晨,郭鹏鹏,周倩,&骆永明.(2018).海岸环境中两类软质微塑料表面生物膜DNA的提取方法比较与优化.海洋学报,40(12),112-119.
MLA 陈涛,et al."海岸环境中两类软质微塑料表面生物膜DNA的提取方法比较与优化".海洋学报 40.12(2018):112-119.

入库方式： OAI收割

来源：烟台海岸带研究所

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