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Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay

文献类型：期刊论文

作者Lv, Enguang; Ding, Jiawang; Qin, Wei
刊名ANALYTICAL CHEMISTRY
出版日期2018-11-20
卷号90期号:22页码:13600-13606
关键词RAPID DETECTION MAGNETIC BEADS RECOGNITION MEMBRANE BACTERIOCINS BIOSENSOR SYSTEM TRIFLUOROETHANOL MECHANISMS ELECTRODES
ISSN号0003-2700
DOI10.1021/acs.analchem.8b03809
产权排序[Lv, Enguang ; Ding, Jiawang ; Qin, Wei] Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China ; [Lv, Enguang ; Ding, Jiawang ; Qin, Wei] Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China ; [Ding, Jiawang ; Qin, Wei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China ; [Lv, Enguang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类Article
英文摘要Peptide-based sandwich assays are promising tools in molecular detection, but may be restricted by the availability of "pairs" of affinity peptides. Herein, a new potentiometric sandwich assay for bacteria based on peptide pairs derived from an antimicrobial peptide (AMP) ligand is demonstrated. As a model, the original AMP with a welldefined structure for Listeria monocytogenes (LM) can be split into two fragments to serve as the peptide pairs for the sandwich assay. The recognition and binding of the short peptide pairs to the target can be verified by circular dichroism, flow cytometry, fluorometry, and optical microscopy. The potentiometric magnetic bead-based sandwich assay is designed by using horseradish peroxidase as a label. The enzyme can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine with H2O2 to induce a potential change on a polymeric membrane ion selective electrode. Under optimal conditions, the concentration of LM can be determined potentiometrically in a linear range of 1.0 X 10(2) to 1.0 x 10(6) CFU mL(-1) with a detection limit of 10 CFU mL(-1) (3 sigma). The proposed sensing strategy expands the applications of peptides in the field of bioassays.
WOS关键词RAPID DETECTION ; MAGNETIC BEADS ; RECOGNITION ; MEMBRANE ; BACTERIOCINS ; BIOSENSOR ; SYSTEM ; TRIFLUOROETHANOL ; MECHANISMS ; ELECTRODES
WOS研究方向Chemistry, Analytical
语种英语
WOS记录号WOS:000451246100062
资助机构National Natural Science Foundation of China [21575158, 21475148, 41876108] ; Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020702] ; Taishan Scholar Program of Shandong Province [TS20081159] ; National Key Research and Development Program of China [2016YFC1400700] ; Key Research and Development Program of Yantai [2018ZHGY053]
源URL[http://ir.yic.ac.cn/handle/133337/24579]  
专题烟台海岸带研究所_近岸生态与环境实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China;
3.Chinese Acad Sci, Shandong Prov Key Lab Coastal Environm Proc, Yantai Inst Coastal Zone Res YIC, Yantai 264003, Shandong, Peoples R China;
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China;
推荐引用方式
GB/T 7714		 Lv, Enguang,Ding, Jiawang,Qin, Wei. Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay[J]. ANALYTICAL CHEMISTRY,2018,90(22):13600-13606.
APA Lv, Enguang,Ding, Jiawang,&Qin, Wei.(2018).Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay.ANALYTICAL CHEMISTRY,90(22),13600-13606.
MLA Lv, Enguang,et al."Potentiometric Detection of Listeria monocytogenes via a Short Antimicrobial Peptide Pair-Based Sandwich Assay".ANALYTICAL CHEMISTRY 90.22(2018):13600-13606.

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来源：烟台海岸带研究所

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