中国科学院机构知识库网格系统: 磷和金属对荣成天鹅湖水体碱性磷酸酶活性和动力学性质的影响研究

磷和金属对荣成天鹅湖水体碱性磷酸酶活性和动力学性质的影响研究

文献类型：期刊论文


作者	吕迎春; 孙志伟 ; 宋佳伟 ; 徐刚
刊名	环境科学学报
出版日期	2018-06-12
卷号	38 期号:10 页码:4083-4089
关键词	碱性磷酸酶动力学特征竞争性抑制天鹅湖
ISSN号	0253-2468
DOI	10.13671/j.hjkxxb.2018.0248
其他题名	Effects of phosphate and metal ions on alkaline phosphatase activity and kinetic parameters in Swan Lake,Rongcheng
产权排序	(1)中国科学院烟台海岸带研究所 ; (2)鲁东大学 ; (3)中国科学院大学
英文摘要	The catalytic hydrolysis of organic phosphorus by phosphatase represents an important source of available phosphorus in surface water. To assess the effects of phosphate and metal ions on alkaline phosphatase activity and kinetic parameters,surface water samples were taken from the coastal Swan Lake water in Rongcheng. Results show that phosphate (PO_4~(3-)),hexametaphosphates (PO_3)_6~(6-),pyrophosphate (P_2 O_7~(4-)) and ß-glycerophosphate (C_3H_4O_6P)~(2-)could inhibit the alkaline phosphatase activity (APA) when their concentrations were in the range of 0.01-1mmol·L~(-1). And this inhibition effect increased with the increase of the ion concentrations. In comparison,the APA decreased 50.8%,55.8%,52.4% and 14.4% as treated by 1 mmol·L~(-1) PO_4~(3-),(PO_3)_6~(6-),P_2 O_7~(4-),(C_3 H_4 O_6 P)~(2-),respectively. The activation of APA by Al~(3+)、Co~(2+)、Pb~(2+) and Cr~(6+) increased when their concentration increased. After adding 1 mmol·L~(-1) Al~(3+),Co~(2+),Pb~(2+) and Cr~(6+) to the water,the APA increased by a factor of 2.8,3.1,2.3 and 2.7, respectively. The heavy metal Cu~(2+),Zn~(2+),Ni~(2+) were also found to have inhibition effects on APA,especially for Cu~(2+).The residual APA dramatically decreased to 62.3% at 1 mmol·L~(-1) of Cu~(2+). The significant reduction of an apparent substrate affinity (Km) and the uniform pattern of maximum APA (Vmax) were both observed when the Cu~(2+),PO_4~(3-),(PO_3)_6~(6-),P_2O_7~(4-) increased. This phenomenon indicates that Cu~(2+),PO_4~(3-),(PO_3)_6~(6-),P_2O_7~(4-) behave in competitive inhibition effects. In addition,V_(max) /K_m ratio is a good indicator for the catalytic efficiency of alkaline phosphatases in eutrophic water. This research suggests that the phosphate and metal ions can significantly influence APA and has profound implication for the transform and supplement of the organic phosphorus in eutrophic water.
语种	中文
CSCD记录号	CSCD:6348762
资助机构	国家自然科学基金#41573120 ; 国家自然科学基金#41103073
源URL	[http://ir.yic.ac.cn/handle/133337/24699]
专题	烟台海岸带研究所_近岸生态与环境实验室烟台海岸带研究所_污染过程与控制实验室烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
作者单位	1.中国科学院烟台海岸带研究所; 2.中国科学院大学 3.鲁东大学;
推荐引用方式 GB/T 7714	吕迎春,孙志伟,宋佳伟,等. 磷和金属对荣成天鹅湖水体碱性磷酸酶活性和动力学性质的影响研究[J]. 环境科学学报,2018,38(10):4083-4089.
APA	吕迎春,孙志伟,宋佳伟,&徐刚.(2018).磷和金属对荣成天鹅湖水体碱性磷酸酶活性和动力学性质的影响研究.环境科学学报,38(10),4083-4089.
MLA	吕迎春,et al."磷和金属对荣成天鹅湖水体碱性磷酸酶活性和动力学性质的影响研究".环境科学学报 38.10(2018):4083-4089.

入库方式： OAI收割

来源：烟台海岸带研究所

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磷和金属对荣成天鹅湖水体碱性磷酸酶活性和动力学性质的影响研究

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