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iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium

文献类型：期刊论文

作者Lu, Z; Wang, S; Ji, CL; Li, F; Cong, M; Shan, XJ; Wu, HF
刊名ENVIRONMENTAL POLLUTION
出版日期2020-02
卷号257页码:113591
关键词Cadmium Paralichthys olivaceus iTRAQ Proteomics Mitochondrial toxicity
ISSN号0269-7491
DOI10.1016/j.envpol.2019.113591
产权排序[Lu, Zhen ; Wang, Shuang ; Ji, Chenglong ; Li, Fei ; Cong, Ming ; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China ; [Ji, Chenglong ; Shan, Xiujuan] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China ; [Lu, Zhen ; Wang, Shuang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类Article
英文摘要Cadmium (Cd) is an important heavy metal pollutant in the Bohai Sea. Mitochondria are recognized as the key target for Cd toxicity. However, mitochondria! responses to Cd have not been fully investigated in marine fishes. In this study, the mitochondrial responses were characterized in gills of juvenile flounder Paralichthys olivaceus treated with two environmentally relevant concentrations (5 and 50 mu g/L) of Cd for 14 days by determination of mitochondrial membrane potential (MMP), observation of mitochondrial morphology and quantitative proteomic analysis. Both Cd treatments significantly decreased MMPs of mitochondria from flounder gills. Mitochondrial morphologies were altered in Cd-treated flounder samples, indicated by more and smaller mitochondria. iTRAQ-based proteomic analysis indicated that a total of 128 proteins were differentially expressed in both Cd treatments. These proteins were basically involved in various biological processes in gill mitochondria, including mitochondrial morphology and import, tricarboxylic acid (TCA) cycle, oxidative phosphorylation (OXPHOS), primary bile acid biosynthesis, stress resistance and apoptosis. These results indicated that dynamic regulations of energy homeostasis, cholesterol metabolism, stress resistance, apoptosis, and mitochondrial morphology in gill mitochondria might play significant roles in response to Cd toxicity. Overall, this study provided a global view on mitochondrial toxicity of Cd in flounder gills using iTRAQ-based proteomics. (C) 2019 Elsevier Ltd. All rights reserved.
WOS关键词VIRGINICA GMELIN BIVALVIA ; BILE-ACID SYNTHESIS ; ENERGY-METABOLISM ; ROS PRODUCTION ; COMPLEX-II ; PROTEIN ; MITOFILIN ; ACCUMULATION ; TARGET ; SAM50
WOS研究方向Environmental Sciences
语种英语
WOS记录号WOS:000514746800091
资助机构NSFCNational Natural Science Foundation of China [41676114] ; Young Taishan Scholars Program of Shandong Province [tsqn201812115] ; Qingdao National Laboratory for Marine Science and Technology [QNLM201701]
源URL[http://ir.yic.ac.cn/handle/133337/24756]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_近岸生态与环境实验室
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China;
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China;
推荐引用方式
GB/T 7714		 Lu, Z,Wang, S,Ji, CL,et al. iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium[J]. ENVIRONMENTAL POLLUTION,2020,257:113591.
APA Lu, Z.,Wang, S.,Ji, CL.,Li, F.,Cong, M.,...&Wu, HF.(2020).iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium.ENVIRONMENTAL POLLUTION,257,113591.
MLA Lu, Z,et al."iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium".ENVIRONMENTAL POLLUTION 257(2020):113591.

入库方式： OAI收割

来源：烟台海岸带研究所

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