Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple
文献类型：期刊论文
| 作者 | Jiang, SH; Wang, N; Chen, M ; Zhang, R; Sun, QG; Xu, HF; Zhang, ZY; Wang, YC; Sui, XQ; Wang, SF
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| 刊名 | PLANT BIOTECHNOLOGY JOURNAL
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| 出版日期 | 2020 |
| 关键词 | apple anthocyanin MdMYB1 promoter DNA methylation AGO4 RdDM |
| ISSN号 | 1467-7644 |
| DOI | 10.1111/pbi.13337 |
| 产权排序 | [Jiang, Shenghui ; Wang, Nan ; Sun, Qingguo ; Xu, Haifeng ; Zhang, Zongying ; Wang, Yicheng ; Fang, Hongcheng ; Zuo, Weifang ; Su, Mengyu ; Zhang, Jing ; Chen, Xuesen] Shandong Agr Univ, Coll Hort Sci & Engn, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, State Key Lab Crop Biol, Tai An, Shandong, Peoples R China ; [Chen, Min] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China ; [Zhang, Rui] Heze Univ, Heze, Peoples R China ; [Sui, Xiuqi] Yantai Modern Fruit Ind Dev Co, Yantai Modern Fruit Ind Res Inst, Yantai, Peoples R China ; [Wang, Sufang] Shaanxi Fruit Ind Ctr, Xian, Peoples R China ; [Fei, Zhangjun] Cornell Univ, Boyce Thompson Inst, Ithaca, NY USA |
| 文献子类 | Article; Early Access |
| 英文摘要 | Methylation at the MdMYB1 promoter in apple sports has been reported as a regulator of the anthocyanin pathway, but little is known about how the locus is recognized by the methylation machinery to regulate anthocyanin accumulation. In this study, we analysed three differently coloured 'Fuji' apples and found that differences in the transcript levels of MdMYB1, which encodes a key regulator of anthocyanin biosynthesis, control the anthocyanin content (and therefore colour) in fruit skin. The CHH methylation levels in the MR3 region (-1246 to -780) of the MdMYB1 promoter were found to be negatively correlated with MdMYB1 expression. Thus, they were ideal materials to study DNA methylation in apple sports. The protein of RNA-directed DNA methylation (RdDM) pathway responsible for CHH methylation, MdAGO4, was found to interact with the MdMYB1 promoter. MdAGO4s can interact with MdRDM1 and MdDRM2s to form an effector complex, fulfilling CHH methylation. When MdAGO4s and MdDRM2s were overexpressed in apple calli and Arabidopsis mutants, those proteins increase the CHH methylation of AGO4-binding sites. In electrophoretic mobility shift assays, MdAGO4s were found to specifically bind to sequence containing ATATCAGA. Knockdown of MdNRPE1 did not affect the binding of MdAGO4s to the c3 region of the MdMYB1 promoter in 35S::AGO4 calli. Taken together, our data show that the MdMYB1 locus is methylated through binding of MdAGO4s to the MdMYB1 promoter to regulate anthocyanin biosynthesis by the RdDM pathway. |
| WOS关键词 | MYB TRANSCRIPTION FACTOR ; DIRECTED DNA METHYLATION ; RED COLORATION ; NONCODING RNA ; SKIN COLOR ; FRUIT ; GENE ; EXPRESSION ; ACCUMULATION ; ARGONAUTE4 |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000510084500001 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [31730080, 31701892] ; Ministry of Science and Technology Key Research and Development Plan [SQ2016YFSF030011] ; Agricultural Variety Improvement Project of Shandong Province [2019LZGC007] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24763]  |
| 专题 | 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Shaanxi Fruit Ind Ctr, Xian, Peoples R China; 2.Heze Univ, Heze, Peoples R China; 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai, Peoples R China; 4.Shandong Agr Univ, Coll Hort Sci & Engn, Collaborat Innovat Ctr Fruit & Vegetable Qual & E, State Key Lab Crop Biol, Tai An, Shandong, Peoples R China; 5.Yantai Modern Fruit Ind Dev Co, Yantai Modern Fruit Ind Res Inst, Yantai, Peoples R China; 6.Cornell Univ, Boyce Thompson Inst, Ithaca, NY USA |
| 推荐引用方式 GB/T 7714 | Jiang, SH,Wang, N,Chen, M,et al. Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple[J]. PLANT BIOTECHNOLOGY JOURNAL,2020. |
| APA | Jiang, SH.,Wang, N.,Chen, M.,Zhang, R.,Sun, QG.,...&Chen, XS.(2020).Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple.PLANT BIOTECHNOLOGY JOURNAL. |
| MLA | Jiang, SH,et al."Methylation of MdMYB1 locus mediated by RdDM pathway regulates anthocyanin biosynthesis in apple".PLANT BIOTECHNOLOGY JOURNAL (2020). |
入库方式： OAI收割
来源：烟台海岸带研究所
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