Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis
文献类型：期刊论文
| 作者 | Lv, JS; Zhao, JM ; Yang, DL; Wu, HF; Cong, M
|
| 刊名 | JOURNAL OF INVERTEBRATE PATHOLOGY
 |
| 出版日期 | 2019-09 |
| 卷号 | 166页码:107215 |
| ISSN号 | 0022-2011 |
| 关键词 | Mytilus galloprovincialis Mytimacin-4 Tissue distribution Recombinant proteins Antimicrobial activity |
| DOI | 10.1016/j.jip.2019.107215 |
| 产权排序 | [Lv, Jiasen] Yantai Univ, Biol Sch, Yantai 264005, Peoples R China ; [Zhao, Jianmin ; Yang, Dinglong ; Wu, Huifeng ; Cong, Ming] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Antimicrobial peptides (AMPs) play fundamental roles in the innate immunity of invertebrates. Mytimacin-4 is a kind of AMP gene previously sequenced from Mytilus galloprovincialis based on an identified EST sequence in our lab. In the present study, the tissue distribution and antimicrobial activities of mytimacin-4 were further investigated. A qRT-PCR analysis revealed that mytimacin-4 transcripts were constitutively expressed in all of the tested tissues of M. galloprovincialis, with the highest expression level in the posterior adductor muscle. After challenge by Vibrio anguillarum, the expression level of mytimacin-4 gene was significantly increased at 24 h (P < 0.05) in the mantle and increased at 48 h (P < 0.05) in the posterior adductor muscle. This finding suggested that mytimacin-4 transcripts were inducible upon pathogen infection. A minimal inhibitory concentration (MIC) assay indicated that recombinant mytimacin-4 protein had potent antimicrobial activities against gram-positive and gram-negative bacteria. Among the tested microorganisms, mytimacin-4 protein exhibited strong inhibition activities against Bacillus subtilis and Vibrio parahaemolyticus with MICs of 0.315 mu M and 0.62 mu M respectively. This study provides for the first time direct evidence of antimicrobial action of mytimacin-4 in M. galloprovincialis. |
| WOS关键词 | RICH ANTIMICROBIAL PEPTIDE ; MOLECULAR CHARACTERIZATION ; ANTIBACTERIAL PEPTIDES ; HEMOCYTES ; INVERTEBRATES ; CHALLENGE ; SEAWATER ; IMMUNITY ; BINDING ; LEECH |
| WOS研究方向 | Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000485335900010 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [NSFC 41876135] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24867]  |
| 专题 | 海岸带生物资源高效利用研究与发展中心 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Yantai Univ, Biol Sch, Yantai 264005, Peoples R China; 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Shandong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lv, JS,Zhao, JM,Yang, DL,et al. Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2019,166:107215. |
| APA | Lv, JS,Zhao, JM,Yang, DL,Wu, HF,&Cong, M.(2019).Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis.JOURNAL OF INVERTEBRATE PATHOLOGY,166,107215. |
| MLA | Lv, JS,et al."Tissue distribution and functional characterization of mytimacin-4 in Mytilus galloprovincialis".JOURNAL OF INVERTEBRATE PATHOLOGY 166(2019):107215. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。