Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics
文献类型：期刊论文
| 作者 | Ji, CL ; Lu, Z; Xu, LL; Li, F; Cong, M; Shan, XJ; Wu, HF
|
| 刊名 | ENVIRONMENTAL POLLUTION
 |
| 出版日期 | 2019-08 |
| 卷号 | 251页码:802-810 |
| 关键词 | Cadmium iTRAQ Proteomics Mitochondrial toxicity Ruditapes philippinarum |
| ISSN号 | 0269-7491 |
| DOI | 10.1016/j.envpol.2019.05.046 |
| 产权排序 | [Ji, Chenglong ; Lu, Zhen ; Xu, Lanlan ; Li, Fei ; Cong, Ming ; Wu, Huifeng] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China ; [Ji, Chenglong ; Shan, Xiujuan ; Wu, Huifeng] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China ; [Lu, Zhen ; Xu, Lanlan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Cadmium is one of the most serious metal pollutants in the Bohai Sea. Previous studies revealed that mitochondrion might be the target organelle of Cd toxicity. However, there is a lack of a global view on the mitochondrial responses in marine animals to Cd. In this work, the mitochondrial responses were characterized in clams Ruditapes philippinarum treated with two concentrations (5 and 50 mu g/L) of Cd for 5 weeks using tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining, ultrastructural observation and quantitative proteomic analysis. Basically, a significant decrease of mitochondrial membrane potential (Delta Psi m) was observed in clams treated with the high concentration (50 mu g/L) of Cd. Cd treatments also induced specific morphological changes indicated by elongated mitochondria. Furthermore, iTRAQ-based mitochondrial proteomics showed that a total of 97 proteins were significantly altered in response to Cd treatment. These proteins were closely associated with multiple biological processes in mitochondria, including tricarboxylic acid (TCA) cycle, oxidative phosphorylation, fatty acid beta-oxidation, stress resistance and apoptosis, and mitochondrial fission. These findings confirmed that mitochondrion was one of the key targets of Cd toxicity. Moreover, dynamical regulations, such as reconstruction of energy homeostasis, induction of stress resistance and apoptosis, and morphological alterations, in mitochondria might play essential roles in Cd tolerance. Overall, this work provided a deep insight into the mitochondrial toxicity of Cd in clams based on a global mitochondrial proteomic analysis. (C) 2019 Elsevier Ltd. All rights reserved. |
| WOS关键词 | MUSSELS MYTILUS-GALLOPROVINCIALIS ; VIRGINICA GMELIN BIVALVIA ; SHRIMP CRANGON-AFFINIS ; METABOLIC-RESPONSES ; OXIDATIVE STRESS ; METAL POLLUTION ; MEMBRANE ; INSIGHTS ; FISSION ; BOHAI |
| WOS研究方向 | Environmental Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000474329700088 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41676114] ; Young Taishan Scholars Program of Shandong Province [tsqn201812115] ; Qingdao National Laboratory for Marine Science and Technology [QNLM201701] ; Youth Innovation Promotion Association CAS [2015169] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24896]  |
| 专题 | 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 烟台海岸带研究所_近岸生态与环境实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China; 3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ji, CL,Lu, Z,Xu, LL,et al. Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics[J]. ENVIRONMENTAL POLLUTION,2019,251:802-810. |
| APA | Ji, CL.,Lu, Z.,Xu, LL.,Li, F.,Cong, M.,...&Wu, HF.(2019).Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics.ENVIRONMENTAL POLLUTION,251,802-810. |
| MLA | Ji, CL,et al."Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics".ENVIRONMENTAL POLLUTION 251(2019):802-810. |
入库方式： OAI收割
来源：烟台海岸带研究所
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