Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations
文献类型：期刊论文
| 作者 | Liu, SW; Ding, JW ; Qin, W
|
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019-02-19 |
| 卷号 | 91期号:4页码:3170-3176 |
| 关键词 | ION-SELECTIVE ELECTRODES SOLID-CONTACT LABEL-FREE COLORIMETRIC DETECTION MEMBRANE ELECTRODES APTAMER MOLECULES CYSTEINE SYSTEMS SENSORS |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b05971 |
| 产权排序 | [Liu, Shuwen ; Ding, Jiawang ; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China ; [Liu, Shuwen ; Ding, Jiawang ; Qin, Wei] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China ; [Ding, Jiawang ; Qin, Wei] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China ; [Liu, Shuwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Conventional potentiometric ion sensors that rely on a specific ion carrier in a polymeric membrane can hardly achieve multianalyte detection. Inspired by the remarkable ability of built-in logic gate sensors for multianalyte detection, herein we report a potentiometric aptasensing platform based on a G-quadruplex/hemin DNAzyme and logic gate operations for determination of two analytes using a single membrane electrode. A bifunctional probe with two aptamer units and a signal reporter oligonucleotide with a DNAzyme sequence are assembled on the magnetic beads to form a DNA hybrid structure. The "OR" and "INHIBIT" logic functions can be performed by using the two aptamers and their targets as inputs, and using the chronopotentiometric response based on the G-quadruplex/hemin DNAzyme-H2O2-mediated oxidation of 3,3',5,5'-tetramethylbenzidine as output. Kanamycin and oxytetracycline, as commonly used antibiotics, have been employed as the models and successfully measured. |
| WOS关键词 | ION-SELECTIVE ELECTRODES ; SOLID-CONTACT ; LABEL-FREE ; COLORIMETRIC DETECTION ; MEMBRANE ELECTRODES ; APTAMER ; MOLECULES ; CYSTEINE ; SYSTEMS ; SENSORS |
| WOS研究方向 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000459642400083 |
| 资助机构 | National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21575158, 21475148, 41876108] ; National Key Research and Development Program of China [2016YFC1400700] ; Instrument Developing Project of the Chinese Academy of Sciences [Y728021021] ; Taishan Scholar Program of Shandong Province [TS20081159] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24998]  |
| 专题 | 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266200, Peoples R China; 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu, SW,Ding, JW,Qin, W. Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations[J]. ANALYTICAL CHEMISTRY,2019,91(4):3170-3176. |
| APA | Liu, SW,Ding, JW,&Qin, W.(2019).Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations.ANALYTICAL CHEMISTRY,91(4),3170-3176. |
| MLA | Liu, SW,et al."Dual-Analyte Chronopotentiometric Aptasensing Platform Based on a G-Quadruplex/Hemin DNAzyme and Logic Gate Operations".ANALYTICAL CHEMISTRY 91.4(2019):3170-3176. |
入库方式： OAI收割
来源：烟台海岸带研究所
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