A two-photon ratiometric fluorescent probe for the synergistic detection of the mitochondrial SO2/HClO crosstalk in cells and in vivo
文献类型：期刊论文
| 作者 | Dou, K; Chen, G; Yu, FB; Sun, ZW; Li, GL; Zhao, XE; Chen, LX ; You, JM
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| 刊名 | JOURNAL OF MATERIALS CHEMISTRY B
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| 出版日期 | 2017-11-14 |
| 卷号 | 5期号:42页码:8389-8398 |
| ISSN号 | 2050-750X |
| 关键词 | SULFUR-DIOXIDE DERIVATIVES LIVING CELLS HYPOCHLOROUS ACID OXIDATIVE STRESS HYDROGEN-SULFIDE BIOIMAGING APPLICATIONS PARKINSONS-DISEASE REACTIVE OXYGEN LIVE-CELL TISSUES |
| DOI | 10.1039/c7tb01900a |
| 产权排序 | [Dou, Kun ; Chen, Guang ; Yu, Fabiao ; Sun, Zhiwei ; Li, Guoliang ; Zhao, Xianen ; Chen, Lingxin ; You, Jinmao] Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China ; [Dou, Kun ; Chen, Guang ; Yu, Fabiao ; Chen, Lingxin ; You, Jinmao] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Known as a new member of the reactive sulphur species (RSS), endogenous sulfur dioxide (SO2) plays potentially reductive roles such as antioxidation, anti-aging, anti-inflammation, and cytoprotection; however, SO2 is also a metabolite of antioxidation. Hypochlorous acid (HClO) has powerful bio-effects on the innate immune system, and its uncontrolled production leads to adverse damage in cells. To illuminate the potential crosstalk between SO2 and HClO in redox homeostasis, we designed a two-photon ratiometric fluorescent probe for dual-response to mitochondrial SO2/HClO crosstalk in cells and in vivo. Our probe can effectively achieve ratio-type fluorescence dual-response to SO2 and HClO with desirable properties such as large two-photon absorption cross sections, rapid response times (SO2 , 50 s, HClO < 20 s), high sensitivities (limit of detection 8.0 nM for SO2 and 15.2 nM for HClO), and favorable selectivity. The probe has been successfully applied to quantitatively detect endogenous SO2 and HClO in HeLa and Raw 264.7 cells. We have verified that there exists a crosstalk between SO2 and HClO during the process of oxidative stress in mitochondria. We have also detected that SO2 can be endogenously produced through oxidation of intracellular sulfur-containing amino acids by HClO in zebrafish in real-time. |
| WOS关键词 | SULFUR-DIOXIDE DERIVATIVES ; LIVING CELLS ; HYPOCHLOROUS ACID ; OXIDATIVE STRESS ; HYDROGEN-SULFIDE ; BIOIMAGING APPLICATIONS ; PARKINSONS-DISEASE ; REACTIVE OXYGEN ; LIVE-CELL ; TISSUES |
| WOS研究方向 | Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000414156300011 |
| 资助机构 | National Natural Science Foundation of China [21475074, 21403123, 21375075, 31301595, 21402106, 21475075, 21275089, 21405093, 21405094, 81303179, 21305076] ; Funds of Shandong Province Key Laboratory of Detection Technology for Tumour Markers [KLDTTM2015-6, KLDTTM2015-9] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24346]  |
| 专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China 2.Qufu Normal Univ, Coll Chem & Chem Engn, Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Qufu 273165, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Dou, K,Chen, G,Yu, FB,et al. A two-photon ratiometric fluorescent probe for the synergistic detection of the mitochondrial SO2/HClO crosstalk in cells and in vivo[J]. JOURNAL OF MATERIALS CHEMISTRY B,2017,5(42):8389-8398. |
| APA | Dou, K.,Chen, G.,Yu, FB.,Sun, ZW.,Li, GL.,...&You, JM.(2017).A two-photon ratiometric fluorescent probe for the synergistic detection of the mitochondrial SO2/HClO crosstalk in cells and in vivo.JOURNAL OF MATERIALS CHEMISTRY B,5(42),8389-8398. |
| MLA | Dou, K,et al."A two-photon ratiometric fluorescent probe for the synergistic detection of the mitochondrial SO2/HClO crosstalk in cells and in vivo".JOURNAL OF MATERIALS CHEMISTRY B 5.42(2017):8389-8398. |
入库方式： OAI收割
来源：烟台海岸带研究所
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