Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay
文献类型：期刊论文
| 作者 | Chen, Zhaopeng ; Wang, Han; Zhang, Zhiyang; Chen, Lingxin
|
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2019-01-15 |
| 卷号 | 91期号:2页码:1254-1259 |
| 关键词 | ALKALINE-PHOSPHATASE ACTIVITY SIGNAL AMPLIFICATION PLASMONIC ELISA ORGANIC FRAMEWORKS PEROXIDASE-LIKE IMMUNOASSAY ACID HYDROQUINONE SUBSTRATE DESIGN |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.8b05095 |
| 产权排序 | [Chen, Zhaopeng ; Wang, Han ; Zhang, Zhiyang ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China ; [Chen, Zhaopeng ; Wang, Han ; Zhang, Zhiyang ; Chen, Lingxin] YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China ; [Chen, Zhaopeng] Xian Univ Technol, Sch Sci, Dept Appl Chem, Xian 710061, Shaanxi, Peoples R China ; [Chen, Lingxin] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China ; [Wang, Han ; Zhang, Zhiyang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Herein, a redox-cycling was proposed to amplify the signal of enzyme-linked immunosorbent assay (ELISA), which was performed in a polystyrene microplate based on a classic sandwich-type. After the sandwich immunoreactions were finished, the alkaline phosphatase captured on a microplate triggered the hydrolyzation of i-ascorbic acid 2-phosphate to generate ascorbic acid (AA), which then reduced colorless tris(bathophenanthroline) iron(III) (Fe(BPT)(3)(3+)) encapsulated in the micelle of TX-100 to pink red tris(bathophenanthroline) iron(II) (Fe(BPT)(3)(2+). In the presence of tris(2-carboxyethyl)phosphine, the oxidation product, dehydroascorbic acid, was transformed to AA quickly which then reduced Fe(BPT)(3)(3+) again and again, resulting in the generation of abundant Fe(BPT)(3)(2+) that could be read out conveniently by a commercial microplate reader or the naked eye. Because the negative charged TCEP with large size could hardly pass through the micelle, the reduction of Fe(BPT)(3)(3+) by TCEP directly was negligible. Experiment results for assay of alpha-fetoprotein (a model antigen) showed the cycling greatly improved the detection limit to 5 pg/mL, 2 orders of magnitude lower than that of conventional ELISA. The cycling also exhibited the advantages of simplicity and high reproducibility, implying its great potential for practical applications in biological and clinical diagnosis. |
| WOS关键词 | ALKALINE-PHOSPHATASE ACTIVITY ; SIGNAL AMPLIFICATION ; PLASMONIC ELISA ; ORGANIC FRAMEWORKS ; PEROXIDASE-LIKE ; IMMUNOASSAY ; ACID ; HYDROQUINONE ; SUBSTRATE ; DESIGN |
| WOS研究方向 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000456350000009 |
| 资助机构 | National Natural Science Foundation of China [21575159, 21804010, 41601525, 41776110] ; Natural Science Foundation of Shandong Province of China [ZR2014BL031, ZR2016DB07, ZR2016BL25] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24625]  |
| 专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 烟台海岸带研究所_近岸生态与环境实验室 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.YICCAS, Shandong Prov Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China; 3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China; 4.Xian Univ Technol, Sch Sci, Dept Appl Chem, Xian 710061, Shaanxi, Peoples R China; 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Chen, Zhaopeng,Wang, Han,Zhang, Zhiyang,et al. Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay[J]. ANALYTICAL CHEMISTRY,2019,91(2):1254-1259. |
| APA | Chen, Zhaopeng,Wang, Han,Zhang, Zhiyang,&Chen, Lingxin.(2019).Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay.ANALYTICAL CHEMISTRY,91(2),1254-1259. |
| MLA | Chen, Zhaopeng,et al."Chemical Redox-Cycling for Improving the Sensitivity of Colorimetric Enzyme-Linked Immunosorbent Assay".ANALYTICAL CHEMISTRY 91.2(2019):1254-1259. |
入库方式： OAI收割
来源：烟台海岸带研究所
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