A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model
文献类型：期刊论文
| 作者 | Wang, Y ; Han, XY; Zhang, X; Zhang, L; Chen, LX
|
| 刊名 | ANALYST
 |
| 出版日期 | 2020-02-21 |
| 卷号 | 145期号:4页码:1389-1395 |
| 关键词 | NITROREDUCTASE DETECTION RESISTANCE |
| ISSN号 | 0003-2654 |
| DOI | 10.1039/c9an02436k |
| 产权排序 | [Wang, Yue ; Han, Xiaoyue ; Zhang, Xia ; Zhang, Li ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Wang, Yue ; Zhang, Xia ; Zhang, Li] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China ; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Nitroreductase (NTR) with a high expression level in tumors has been considered as a biomarker of highly aggressive hypoxia tumors. Thus, it is important to develop powerful tools for tumor hypoxia detection. Here, we developed a two-photon fluorescent probe hTP-NNO2 for NTR detection. The probe with one-step synthesis exhibited high yield. hTP-NNO2 showed high selectivity and sensitivity for NTR and the detection limit was as low as 43 ng mL(-1). hTP-NNO2 also showed low cytotoxicity and high stability, indicating that hTP-NNO2 is suitable for NTR detection in real-time and in situ under physiological conditions. hTP-NNO2 was used for NTR imaging in hypoxia cells and the fluorescence intensity of hTP-NNO2 increased with decreasing oxygen concentration. Benefiting from the advantages of two-photon fluorescent probes, we performed NTR detection in deep brain tissue with an imaging depth of up to 100 mu m. hTP-NNO2 was further successfully applied for NTR detection in zebrafish and tumors. These results indicated that we developed a promising fluorescence imaging tool for NTR detection in vitro and in vivo. |
| WOS研究方向 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000515832700030 |
| 资助机构 | National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21976209, 21575159, 21778026] ; Taishan Scholar Project |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24748]  |
| 专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_中科院烟台海岸带研究所知识产出 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; 4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Y,Han, XY,Zhang, X,et al. A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model[J]. ANALYST,2020,145(4):1389-1395. |
| APA | Wang, Y,Han, XY,Zhang, X,Zhang, L,&Chen, LX.(2020).A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model.ANALYST,145(4),1389-1395. |
| MLA | Wang, Y,et al."A high-selectivity fluorescent probe for hypoxia imaging in cells and a tumor-bearing mouse model".ANALYST 145.4(2020):1389-1395. |
入库方式： OAI收割
来源：烟台海岸带研究所
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