Glutathione Peroxidase-Activatable Two-Photon Ratiometric Fluorescent Probe for Redox Mechanism Research in Aging and Mercury Exposure Mice Models
文献类型：期刊论文
| 作者 | Wang, Y ; Zhang, LW; Chen, LX
|
| 刊名 | ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2020-01-21 |
| 卷号 | 92期号:2页码:1997-2004 |
| 关键词 | IN-VIVO SELENOL SELENOCYSTEINE SELENOPROTEIN NEUROTOXICITY METABOLISM SPECIATION REDUCTASE PROTEINS STRESS |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.9b04381 |
| 产权排序 | [Wang, Yue ; Zhang, Liangwei ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China ; [Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China ; [Zhang, Liangwei ; Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China ; [Wang, Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Chen, Lingxin] Qufu Normal Univ, Dept Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Solid evidence confirms that glutathione peroxidase (GPx) is a kind of vital protease in the first-line antioxidant defense system and participates in regulation of redox homeostasis as well as the pentose phosphate pathway. However, the current methods cannot achieve real-time and in situ visualization studies of GPx. In addition, GPx is highly reactive and susceptible to external interference, and there is rare research for exploring the roles of GPx under environmental factor exposure. Herein, we report a novel two-photon ratiometric fluorescent probe (TP-SS) for GPx detection for the first time. Using TP-SS, we explore the reversible catalytic cycle and the antioxidant mechanisms of GPx/GSH redox pool in aging and mercury exposure models. We detect the concentration fluctuation of GPx in aging and mercury exposure mice models. Also, we perform GPx detection in deep brain tissue and the imaging depth up to 100 mu m. We believe that the novel two-photon ratiometric fluorescent probe TP-SS can facilitate the development of GPx-targeting tools and offer great advances in exploring the physiological/pathological functions of GPx. |
| WOS关键词 | IN-VIVO ; SELENOL ; SELENOCYSTEINE ; SELENOPROTEIN ; NEUROTOXICITY ; METABOLISM ; SPECIATION ; REDUCTASE ; PROTEINS ; STRESS |
| WOS研究方向 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000509420400047 |
| 资助机构 | National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21976209, 21778026, 21575159] ; program of Youth Innovation Promotion Association, CAS [2019217] ; Taishan Scholar Project Special Funding [ts20190962] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24765]  |
| 专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 2.Qufu Normal Univ, Dept Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China; 4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; 5.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Y,Zhang, LW,Chen, LX. Glutathione Peroxidase-Activatable Two-Photon Ratiometric Fluorescent Probe for Redox Mechanism Research in Aging and Mercury Exposure Mice Models[J]. ANALYTICAL CHEMISTRY,2020,92(2):1997-2004. |
| APA | Wang, Y,Zhang, LW,&Chen, LX.(2020).Glutathione Peroxidase-Activatable Two-Photon Ratiometric Fluorescent Probe for Redox Mechanism Research in Aging and Mercury Exposure Mice Models.ANALYTICAL CHEMISTRY,92(2),1997-2004. |
| MLA | Wang, Y,et al."Glutathione Peroxidase-Activatable Two-Photon Ratiometric Fluorescent Probe for Redox Mechanism Research in Aging and Mercury Exposure Mice Models".ANALYTICAL CHEMISTRY 92.2(2020):1997-2004. |
入库方式： OAI收割
来源：烟台海岸带研究所
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