Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative
文献类型：期刊论文
| 作者 | Gao, M ; Zhang, X; Wang, Y; Liu, QL; Yu, FB; Huang, Y; Ding, CF; Chen, LX
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| 刊名 | ANALYTICAL CHEMISTRY
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| 出版日期 | 2019-06-18 |
| 卷号 | 91期号:12页码:7774-7781 |
| 关键词 | REACTIVE OXYGEN SULFANE SULFUR LIVING CELLS OXIDATIVE STRESS CHEMICAL PROBES ON PROBE SULFIDE NITROREDUCTASE LUMINESCENT PEROXYNITRITE |
| ISSN号 | 0003-2700 |
| DOI | 10.1021/acs.analchem.9b01189 |
| 产权排序 | [Gao, Min ; Zhang, Xia ; Wang, Yue ; Huang, Yan ; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China ; [Yu, Fabiao] Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China ; [Liu, Qingluan] China Med Univ, Div Clin Med 3, Shenyang 110122, Liaoning, Peoples R China ; [Ding, Caifeng] Qingdao Univ Sci & Technol, MOE, Key Lab Opt Electr Sensing & Analyt Chem Life Sci, Qingdao 266042, Shandong, Peoples R China ; [Ding, Caifeng] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Shandong, Peoples R China ; [Gao, Min ; Zhang, Xia ; Wang, Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China ; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Chronic hypoxic stress disrupts the intracellular redox homeostasis, leads to a series of physiological dysfunction, and finally results in many diseases including cancer and inflammatory and cardiovascular diseases. The intracellular redox status is related to the homeostasis between reactive oxygen species (ROS) and cellular antioxidant species. Superoxide anion (O-2(center dot-) is considered to be a precursor of ROS. As a member of reactive sulfur species, hydrogen polysulfides (H2Sn) are a class of antioxidants in cells, which act as an important regulator for the intracellular redox state. Therefore, trapping the cross-talk of O-2(center dot-) and H2Sn is a benefit for further understanding the physiological and pathological effects. Herein, we conceive a fluorescent probe HCy-ONO for sequential detection of O-2(center dot-) and H2Sn in cells and in mouse models. Based on a tandem reaction, the probe HCy-ONO can be used to detect O-2(center dot-) and H2Sn in different fluorescence collection windows without spectral overlap interference with limits of detection 90 and 100 nM, respectively. The strategy affords high sensitivity and selectivity for our detection in living cell models under continuous hypoxic and intermittent hypoxic conditions, revealing the reason for ischemia-reperfusion injury. Moreover, the probe can distinguish the inflamed tissue from normal tissue in acute peritonitis mouse model. Finally, our probe is successfully applied for imaging of O-2(center dot-) and H2Sn in the SH-SYSY tumor-bearing mouse model, which is helpful to elucidate the physiological and pathological processes. These data demonstrated that different hypoxic status lead to different concentrations between H2Sn and O-2(center dot-). |
| WOS关键词 | REACTIVE OXYGEN ; SULFANE SULFUR ; LIVING CELLS ; OXIDATIVE STRESS ; CHEMICAL PROBES ; ON PROBE ; SULFIDE ; NITROREDUCTASE ; LUMINESCENT ; PEROXYNITRITE |
| WOS研究方向 | Chemistry, Analytical |
| 语种 | 英语 |
| WOS记录号 | WOS:000472682000037 |
| 资助机构 | National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 21775162, 41776110] ; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CASChinese Academy of Sciences [KF2016-22] ; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24918]  |
| 专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 烟台海岸带研究所_污染过程与控制实验室 烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室 |
| 作者单位 | 1.Qingdao Univ Sci & Technol, MOE, Key Lab Opt Electr Sensing & Analyt Chem Life Sci, Qingdao 266042, Shandong, Peoples R China; 2.China Med Univ, Div Clin Med 3, Shenyang 110122, Liaoning, Peoples R China; 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; 4.Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China; 5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 7.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Shandong, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Gao, M,Zhang, X,Wang, Y,et al. Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative[J]. ANALYTICAL CHEMISTRY,2019,91(12):7774-7781. |
| APA | Gao, M.,Zhang, X.,Wang, Y.,Liu, QL.,Yu, FB.,...&Chen, LX.(2019).Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative.ANALYTICAL CHEMISTRY,91(12),7774-7781. |
| MLA | Gao, M,et al."Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative".ANALYTICAL CHEMISTRY 91.12(2019):7774-7781. |
入库方式： OAI收割
来源：烟台海岸带研究所
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