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A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum

文献类型：期刊论文

作者Zhang, JN; Zhang, YF; Zhang, LB; Wei, QY; Liu, XL; Yang, DL
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2019-11
卷号94页码:72-80
关键词Ruditapes philippinarum Sialic acid-binding lectin Pattern recognition receptor Immune recognition
ISSN号1050-4648
DOI10.1016/j.fsi.2019.08.074
产权排序[Zhang, Jianning ; Zhang, Yifei ; Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China ; [Liu, Xiaoli] Ludong Univ, Coastal Resources & Environm Team Blue Yellow Are, Yantai 264025, Peoples R China ; [Zhang, Linbao] Chinese Acad Fisheries Sci, Guangdong Prov Key Lab Fishery Ecol & Environm, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China ; [Yang, Dinglong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China ; [Wei, Qianyu ; Yang, Dinglong] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
文献子类Article
英文摘要In the present study, a sialic acid-binding lectin was cloned and characterized from Manila clam Ruditapes philippinarum (designed as RpSabl). The open reading frame of RpSabl encoded a polypepdde of 162 amino acids with a calculated molecular mass of 17.7 kDa. Analysis of the conserved domain suggested that RpSabl was a new member of the sialic acid-binding lectins family. In non-stimulated clams, RpSabl transcripts were constitutively expressed in all five tested tissues, especially in hepatopancreas. After Vibrio anguillarum challenge, the expression of RpSabl mRNA in hepatopancreas was significantly up-regulated at 3 h (3.8-fold, P < 0.05), 6 h (4.9-fold, P < 0.05), 12 h (12.3-fold, P < 0.01) and 24 h (9.7-fold, P < 0.01), while RpSabl transcripts in hemocytes was only significantly up-regulated at 6h (8.5-Fold, P < 0.01). MAI-mediated knockdown of RpSabl transcripts affected the survival rates of Manila clam against V. anguillarum, perhaps mainly due to the inhibited expression of antibacterial effectors (e.g. lysozyme and defensin). Moreover, recombinant protein of RpSabl (rRpSabl) possessed binding activities towards lipopolysaccharides (LPS), peptidoglycan (PGN) and glucan in vitro. Coinciding with the Pathogen-associated molecular patterns (PAMPs) binding assay, rRpSabl displayed broad bacterial-agglutination properties towards Vibrio harveyi, Vibrio splendidus, V. anguillarum, Enterobacter cloacae and Aeromonas hydrophila. Meanwhile, the phagocytosis and encapsulation ability of hemocytes could be significantly enhanced by rRpSabl incubation. All these results showed that RpSabl could function as a versatile molecule involved in the innate immune responses of R. philippinarum.
WOS关键词C1Q-DOMAIN-CONTAINING PROTEIN ; ANTIBACTERIAL ACTIVITY ; IMMUNE ; COMPLEMENT ; C1Q ; RECOGNITION ; GENERATION ; MOLECULES ; PEPTIDE ; SABLS
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000496892400009
资助机构Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KFJ-STS-ZDTP-023] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022] ; Guangdong Provincial Key Laboratory of Fishery Ecology and Environment [LFE-2016-6] ; Youth Innovation Promotion Association of CAS [2016196]
源URL[http://ir.yic.ac.cn/handle/133337/24821]  
专题中国科学院牟平海岸带环境综合试验站
海岸带生物资源高效利用研究与发展中心
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China;
2.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China;
3.Ludong Univ, Coastal Resources & Environm Team Blue Yellow Are, Yantai 264025, Peoples R China;
4.Chinese Acad Fisheries Sci, Guangdong Prov Key Lab Fishery Ecol & Environm, South China Sea Fisheries Res Inst, Guangzhou 510300, Guangdong, Peoples R China;
5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, JN,Zhang, YF,Zhang, LB,et al. A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2019,94:72-80.
APA Zhang, JN,Zhang, YF,Zhang, LB,Wei, QY,Liu, XL,&Yang, DL.(2019).A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,94,72-80.
MLA Zhang, JN,et al."A sialic acid-binding lectin with bactericidal and opsonic activities from Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 94(2019):72-80.

入库方式： OAI收割

来源：烟台海岸带研究所

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