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Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization

文献类型：期刊论文

作者Zhang, JN; Zhang, YF; Chen, LZ; Yang, JM; Wei, QY; Yang, BW; Liu, XL; Yang, DL
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2019-11
卷号94页码:230-238
关键词Venerupis philippinarum c-type lectin Pattern recognition receptor Immune recognition
ISSN号1050-4648
DOI10.1016/j.fsi.2019.09.009
产权排序[Zhang, Jianning ; Zhang, Yifei ; Yang, Jianmin ; Yang, Bowen ; Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China ; [Wei, Qianyu ; Yang, Dinglong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Moping Coastal Environm Res Stn, Yantai 264003, Peoples R China ; [Yang, Dinglong] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China ; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
文献子类Article
英文摘要In the study, two c type lectins were identified and characterized from the manila clam Venerupis philippinarum (designed as VpClec-1 and VpClec-2, respectively). Multiple alignments and phylogenetic analysis strongly suggested that they were new members of the c-type lectin superfamily. In normal tissue of clams, both VpClec-1 and VpClec-2 transcripts were highly expressed in the tissue of hepatopancreas. After Vibrio anguillartun challenge, the temporal expression of both VpClec-1 and VpClec-2 transcripts was up-regulated in the hemocytes of manila clams. The recombinant protein VpClec-1 (rVpClec-1) showed obvious binding activities to lipopolysaccharide (LPS), peptidoglycan (PGN), glucan and zymosan in vitro, while the recombinant protein VpClec-2 (rVpClec-2) could only bind LPS, glucan and zymosan. Coinciding with the PAMPs binding assay, both rVpClec-1 and rVpClec-2 displayed broad agglutination and antibacterial activities towards Vibrio harveyi, Vibrio splendidus, Vibrio arquillarum, Enterobacter cloacae and Aeromonas hydrophila. Moreover, the phagocytosis and encapsulation ability of hemocytes could be significantly enhanced by rVpClec-1 and rVpClec-2. Notably, the rVpClec-1 but not rVpClec-2 elicited a chemotactic response from hemocytes. All the results showed that VpClec-1 and VpClec-2 functioned as pattern recognition receptors (PRRs) with distinct recognition spectrum, and involved in the innate immune responses of manila clams.
WOS关键词ACID-BINDING LECTIN ; MOLECULAR-CLONING ; LITOPENAEUS-VANNAMEI ; MANDUCA-SEXTA ; PROTEIN ; GENE ; DOMAIN ; PEPTIDOGLYCAN ; IMMULECTIN-2 ; ACTIVATION
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000496892400025
资助机构Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196, 41506190] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022] ; 13th Five-year Informatization Plan of Chinese Academy of Sciences [XXH13506]
源URL[http://ir.yic.ac.cn/handle/133337/24822]  
专题中国科学院牟平海岸带环境综合试验站
海岸带生物资源高效利用研究与发展中心
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Moping Coastal Environm Res Stn, Yantai 264003, Peoples R China;
2.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China;
3.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
4.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China;
推荐引用方式
GB/T 7714		 Zhang, JN,Zhang, YF,Chen, LZ,et al. Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization[J]. FISH & SHELLFISH IMMUNOLOGY,2019,94:230-238.
APA Zhang, JN.,Zhang, YF.,Chen, LZ.,Yang, JM.,Wei, QY.,...&Yang, DL.(2019).Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization.FISH & SHELLFISH IMMUNOLOGY,94,230-238.
MLA Zhang, JN,et al."Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization".FISH & SHELLFISH IMMUNOLOGY 94(2019):230-238.

入库方式： OAI收割

来源：烟台海岸带研究所

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