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A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode

文献类型：期刊论文

作者Yang, DL; Han, YJ; Chen, LZ; Cao, RW; Wang, Q; Dong, ZJ; Liu, H; Zhang, XL; Zhang, QQ; Zhao, JM
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2019-09
卷号92页码:897-904
关键词Venerupis philippinarum Macin Molecular characterization Antimicrobial activity
ISSN号1050-4648
DOI10.1016/j.fsi.2019.07.031
产权排序[Yang, Dinglong ; Han, Yijing ; Cao, Ruiwen ; Wang, Qing ; Dong, Zhijun ; Liu, Hui ; Zhang, Xiaoli ; Zhang, Qianqian ; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China ; [Yang, Dinglong ; Wang, Qing ; Dong, Zhijun ; Liu, Hui ; Zhang, Xiaoli ; Zhang, Qianqian ; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China ; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China ; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
文献子类Article
英文摘要In the present study, a macin was cloned and characterized from clam Venerupis philippinarum (designed as VpMacin). The full-length cDNA of VpMacin was of 579 bp, encoding a peptide of 87 amino acids with the predicted molecular weight of 9.7 kDa. Analysis of the conserved domain suggested that VpMacin was a new member of the macin family. In non-stimulated clams, VpMacin transcripts exhibited different tissue expression pattem, and highly expressed in the tissues of gills and hepatopancreas. Generally, the temporal expression of VpMacin transcripts was significantly induced in hemocytes of clams post Vibrio anguillarum challenge. Moreover, the recombinant VpMacin protein (rVpMacin) showed obvious antimicrobial activities against Grampositive and Gram-negative bacteria. After incubated with 40 mu M rVpMacin, all detected Escherichia coli could be killed within 60 min. Membrane integrity analysis revealed that rVpMacin could increase the membrane permeability of bacteria and then resulted in cell death. Overall, our results suggested that VpMacin had an important function in host defense against invasive pathogens.
WOS关键词CLAM RUDITAPES-PHILIPPINARUM ; CATIONIC ANTIMICROBIAL PEPTIDES ; MOLECULAR CHARACTERIZATION ; INNATE IMMUNITY ; VIBRIO-TAPETIS ; DEFENSIN ; MECHANISMS ; EXPRESSION ; FAMILY ; INVOLVEMENT
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000483634300097
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41476126, 41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KFJ-STS-ZDTP-023] ; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022] ; Youth Innovation Promotion Association of CAS [2016196]
源URL[http://ir.yic.ac.cn/handle/133337/24873]  
专题中国科学院牟平海岸带环境综合试验站
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
海岸带生物资源高效利用研究与发展中心
作者单位1.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China;
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China;
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China;
推荐引用方式
GB/T 7714		 Yang, DL,Han, YJ,Chen, LZ,et al. A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode[J]. FISH & SHELLFISH IMMUNOLOGY,2019,92:897-904.
APA Yang, DL.,Han, YJ.,Chen, LZ.,Cao, RW.,Wang, Q.,...&Zhao, JM.(2019).A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode.FISH & SHELLFISH IMMUNOLOGY,92,897-904.
MLA Yang, DL,et al."A macin identified from Venerupis philippinarum: Investigation on antibacterial activities and action mode".FISH & SHELLFISH IMMUNOLOGY 92(2019):897-904.

入库方式： OAI收割

来源：烟台海岸带研究所

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