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A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum

文献类型：期刊论文

作者Yang, DL; Han, YJ; Liu, YL; Cao, RW; Wang, Q; Dong, ZJ; Liu, H; Zhang, XL; Zhang, QQ; Zhao, JM
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2019-05
卷号88页码:441-448
关键词Ruditapes philippinarum Peptidoglycan recognition protein Immune recognition Immune response
ISSN号1050-4648
DOI10.1016/j.fsi.2019.03.017
产权排序[Yang, Dinglong ; Han, Yijing ; Liu, Yongliang ; Cao, Ruiwen ; Wang, Qing ; Dong, Zhijun ; Liu, Hui ; Zhang, Xiaoli ; Zhang, Qianqian ; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China ; [Yang, Dinglong ; Han, Yijing ; Wang, Qing ; Dong, Zhijun ; Liu, Hui ; Zhang, Xiaoli ; Zhang, Qianqian ; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China ; [Zhao, Jianmin] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China ; [Han, Yijing ; Cao, Ruiwen] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
文献子类Article
英文摘要Peptidoglycan recognition proteins (PGRPs) are important pattern recognition receptors in the innate immune system of invertebrates. In the study, a short PGRP (designed as RpPGRP) was identified and characterized from the manila clam Ruditapes philippinamm. The open reading frame of RpPGRP encoded a polypeptide of 249 amino acids with a calculated molecular mass of 27.2 kDa and an isoelectric point of 6.62. Multiple alignments and phylogenetic analysis strongly suggested that RpPGRP was a new member of the PGRP superfamily. In non stimulated clams, RpPGRP exhibited different tissue expression pattern, and highly expressed in hepatopancreas and hemocytes. Expression of RpPGRP transcripts was significantly up -regulated in hemocytes of clams post Vibrio crnguillartzn or Micrococcus luteus challenge. The recombinant RpPGRP (rRpPGRP) exhibited high affinity to PGN, LPS and zymosan in a concentration-dependent manner. With a broad spectrum of bacterial binding activities, rRpPGRP exhibited strong agglutination activity to Escherichia colt, Vibrio splendidus, V. anguiliarum and M. luteus. Furthermore, rRpPGRP exhibited Zn2+ -dependent amidase activity and catalyzed the degradation of insoluble PGN. Especially, rRpPGRP exhibited significant antibacterial activity against E. coil and M. luteus. Moreover, the biofilm formation of E. coil could be inhibited after rRpPGRP incubation in the presence of Zn2+. This inhibitory effect of rRpPGRP might attribute to its amide bactericidal activity. Taken together, rRpPGRP played important roles in PGRP-mediated immune defense mechanisms, especially by recognizing antigens and eliminating bacteria.
WOS关键词GRAM-NEGATIVE BACTERIA ; L-ALANINE AMIDASE ; MOLECULAR-CLONING ; BINDING PROTEIN ; RECEPTOR ; GENES ; LIPOPOLYSACCHARIDE ; IDENTIFICATION ; PURIFICATION ; EXPRESSION
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000465060500052
资助机构Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41476126, 41806196] ; Key Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [KZZD-EW-14, KFJ-STS-ZDTP-023] ; Two-Hundred Talents Plan of Yantai [Y839081021] ; Youth Innovation Promotion Association CAS [2016196]
源URL[http://ir.yic.ac.cn/handle/133337/24948]  
专题海岸带生物资源高效利用研究与发展中心
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
中国科学院牟平海岸带环境综合试验站
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China;
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai 264003, Peoples R China;
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China;
推荐引用方式
GB/T 7714		 Yang, DL,Han, YJ,Liu, YL,et al. A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum[J]. FISH & SHELLFISH IMMUNOLOGY,2019,88:441-448.
APA Yang, DL.,Han, YJ.,Liu, YL.,Cao, RW.,Wang, Q.,...&Zhao, JM.(2019).A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum.FISH & SHELLFISH IMMUNOLOGY,88,441-448.
MLA Yang, DL,et al."A peptidoglycan recognition protein involved in immune recognition and immune defenses in Ruditapes philippinarum".FISH & SHELLFISH IMMUNOLOGY 88(2019):441-448.

入库方式： OAI收割

来源：烟台海岸带研究所

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