产甲烷分离物中Clostridium spp.与Methanosarcina barkeri潜在的种间直接电子传递
文献类型：期刊论文
| 作者 | 李莹; 郑世玲; 张洪霞; 王炳臣; 王欧美; 刘芳华
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| 刊名 | 微生物学通报
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| 卷号 | 44期号:3页码:591-600 |
| 关键词 | Clostridium spp. Geobacter Methanosarcina barkeri Direct interspecies electron transfer (DIET) Gram-positive bacterium Clostridium spp. Geobacter Methanosarcina barkeri 种间直接电子传递 革兰氏阳性菌 |
| ISSN号 | 0253-2654 |
| DOI | DOI: 10.13344/j.microbiol.china.160218 |
| 其他题名 | Potential direct interspecies electron transfer (DIET) from Clostridium spp. to Methanosarcina barkeri in methanogenic isolates |
| 产权排序 | (1)中国科学院烟台海岸带研究所 海岸带生物学与生物资源利用重点实验室 |
| 文献子类 | Article |
| 英文摘要 | [Objective] Geobacter metallireducens, a Gram-negative bacterium, can directly transfer electron to acetoclastic methanogens such as Methanosaeta harundinacea and Methanosarcina barkeri for reducing carbon dioxide to methane. Our previous results showed that Methanosarcina mazei and Geobacteraceae formed aggregates in an iron(III)-reducing enrichment culture indicating direct interspecies electron transfer. However, the capability of direct electron transfer with methanogens for Gram-positive iron(III)-reducing bacteria such as Clostridium spp. is still unknown. [Methods] In this further study, methanogenic isolates(S6)were achieved from the iron(III)-reducing enrichment by roll-tube(Hungate)method with ethanol as the sole electron donor. We used terminal restriction fragment length polymorphism(T-RFLP)and clone library analysis to investigate the community of S6 and used electrochemical method such as cyclic voltammetry(CV)to confirm the electroactivity of isolates. [Results] Clone library analysis of 16S rRNA gene showed that Clostridium spp.(close to C. tunisiense)and Methanosarcina barkeri were predominant in the bacterial and archaeal community, respectively. Interestingly, addition of G. metallireducens into S6 did not increase the ability of both iron(III)reduction and methanogenesis, indicating that Clostridium spp. may play a similar role in direct interspecies electron transfer from G. metallireducens to M. barkeri. Furthermore, current generation of the S6 suspension dramatically decreased when contact between the organisms and the electrodes was prevented by dialysis bag, and CV showed no obvious redox peaks. [Conclusion] These results suggested that there was direct electron transfer in the methanogenic isolates. This work demonstrated that the dominant Gram-positive Clostridium spp. can potentially directly transfer electron to M. barkeri in methanogenic isolates. |
| WOS关键词 | Microbiology |
| WOS研究方向 | Microbiology ; 微生物学 |
| 语种 | 中文 |
| CSCD记录号 | CSCD:5938961 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24256]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 中国科学院烟台海岸带研究所 海岸带生物学与生物资源利用重点实验室 |
| 推荐引用方式 GB/T 7714 | 李莹,郑世玲,张洪霞,等. 产甲烷分离物中Clostridium spp.与Methanosarcina barkeri潜在的种间直接电子传递[J]. 微生物学通报,44(3):591-600. |
| APA | 李莹,郑世玲,张洪霞,王炳臣,王欧美,&刘芳华. |
| MLA | 李莹,et al."产甲烷分离物中Clostridium spp.与Methanosarcina barkeri潜在的种间直接电子传递".微生物学通报 44.3 |
入库方式： OAI收割
来源：烟台海岸带研究所
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