中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
茎瘤固氮根瘤菌甲基化受体蛋白Tlp1的功能

文献类型:期刊论文

作者杨金保; 刘卫; 姜南; 吴海龙; 李岩; 李润植; 张红梅; 解志红
刊名微生物学通报
出版日期2017-11-28
卷号44期号:3页码:648-654
ISSN号0253-2654
关键词Azorhizobium caulinodans ORS571 Chemotaxis Melanin Nitrogenase activity 茎瘤固氮根瘤菌ORS571 趋化 黑色素 固氮酶活性
DOIDOI:10.13344/j.microbiol.china.160270
其他题名Function of a methyl-accepting chemotaxis protein Tlp1 in Azorhizobium caulinodans ORS571
产权排序(1) 山西农业大学农学院 ; (2) 中国科学院烟台海岸带研究所
文献子类Article
英文摘要[Objective] To study the mutation phenotype of the methyl-accepting chemotaxis protein Tlp1(transducer-like protein)located in the upstream of the gene cluster of Azorhizobium caulinodans ORS571, and to explore its functional mechanism. [Methods] The tlp1 mutant strain was constructed by the homologous recombination and triparental conjugation. Growth of the wild-type strain ORS571 and the tlp1 mutant was compared by measuring OD_(600) over time of cultures in TY medium. The semi-solid plate assay was used to test the ability of chemotaxis. The production of extracellular polysaccharide and secondary metabolites was measured through Congo red solid culture medium. The acetylene reduction assay was used to measure nitrogen fixation ability. [Results] Compared with the wild type, the growth of the mutant in rich media was the same as that of the wild type. The tlp1 mutant was impaired in chemotaxis to glycerol, and the complemented strain's chemotactic capability could be partially complemented. The production of extracellular polysaccharides secreted from tlp1 mutant and wild type has no difference, but one of the secondary metabolites-the melanin tlp1 mutant appeared earlier than the wild type. The nitrogenase activity of tlp1 mutant strain was weaker than that of the wild type, and the complemented strain could complement part of the nitrogen fixation ability. [Conclusion] The methyl-accepting chemotaxis protein Tlp1 of A. caulinodans ORS571 showed a certain ability of chemotaxis to glycerol, and also affected the secondary metabolites and the nitrogenase activity in ORS571.
WOS关键词Microbiology
WOS研究方向Microbiology ; 微生物学
语种中文
CSCD记录号CSCD:5938967
源URL[http://ir.yic.ac.cn/handle/133337/24285]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.中国科学院烟台海岸带研究所
2.山西农业大学农学院;
推荐引用方式
GB/T 7714
杨金保,刘卫,姜南,等. 茎瘤固氮根瘤菌甲基化受体蛋白Tlp1的功能[J]. 微生物学通报,2017,44(3):648-654.
APA 杨金保.,刘卫.,姜南.,吴海龙.,李岩.,...&解志红.(2017).茎瘤固氮根瘤菌甲基化受体蛋白Tlp1的功能.微生物学通报,44(3),648-654.
MLA 杨金保,et al."茎瘤固氮根瘤菌甲基化受体蛋白Tlp1的功能".微生物学通报 44.3(2017):648-654.

入库方式: OAI收割

来源:烟台海岸带研究所

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