中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
凯氏拟小球藻己糖激酶基因的克隆及其在不同培养条件下的表达分析

文献类型:期刊论文

作者陈军; 崔红利; 赵佳琳; 秦松
刊名海洋科学
出版日期2017-07-15
卷号41期号:7页码:1-8
关键词Parachlorella kessleri hexokinase molecular clone transcript expression 凯氏拟小球藻(Parachlorella kessleri) 己糖激酶 基因克隆 表达分析
ISSN号1000-3096
DOIDOI: 10.11759/hykx20170225001
其他题名Molecular cloning of hexokinase from Parachlorella kessleri and its expression analysis under different trophic modes
产权排序(1) 中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室 ; (2) 中国科学院大学 ; (3) 大连大学生命科学与技术学院
文献子类Article
英文摘要cDNA (GenBank ID: AHF54566) encoding hexokinase (termed CkeHK) from the green alga Parachlorella kessleri was cloned and sequenced to understand the response to the presence and uptake of glucose at the molecular level. The transcriptional expression patterns of CkeHK were observed under phototrophic, heterotrophic, and mixotrophic culture conditions. The results indicated that the CkeHk cDNA was 1, 844 base pairs (bp) long, with an open reading frame (ORF) of 1 389 bp encoding 462 amino acids, with a calculated molecular mass of 49.73 kDa and an estimated isoelectric point of 6.98. Under the phototrophic condition as control, the transcriptional profiles of CkeHk showed that it could be upregulated under the heterotrophic and mixotrophic culture conditions. The mRNA level of CkeHK under the mixotrophic culture condition could be upregulated more than that under the heterotrophic culture condition. These results suggest that light may play an important role in regulating the role of hexokinase in metabolizing exogenous glucose. These findings provide us valuable information for exploring the mechanism of metabolizing glucose in P. kessleri.
WOS关键词Genetics & Heredity
WOS研究方向Genetics & Heredity ; 遗传与遗传
语种中文
CSCD记录号CSCD:6105497
源URL[http://ir.yic.ac.cn/handle/133337/24286]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室;
2.大连大学生命科学与技术学院
3.中国科学院大学;
推荐引用方式
GB/T 7714
陈军,崔红利,赵佳琳,等. 凯氏拟小球藻己糖激酶基因的克隆及其在不同培养条件下的表达分析[J]. 海洋科学,2017,41(7):1-8.
APA 陈军,崔红利,赵佳琳,&秦松.(2017).凯氏拟小球藻己糖激酶基因的克隆及其在不同培养条件下的表达分析.海洋科学,41(7),1-8.
MLA 陈军,et al."凯氏拟小球藻己糖激酶基因的克隆及其在不同培养条件下的表达分析".海洋科学 41.7(2017):1-8.

入库方式: OAI收割

来源:烟台海岸带研究所

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