利用多功能裂合酶CpcE/F合成非天然重组藻胆蛋白
文献类型：期刊论文
| 作者 | 吝晓君; 王祥法; 葛保胜; 秦松
|
| 刊名 | 海洋科学
 |
| 出版日期 | 2017-03-15 |
| 卷号 | 41期号:3页码:68-74 |
| 关键词 | phycocybiliproteins biosynthesis unnatural CpcE/Flyase 藻胆蛋白 生物合成 非天然 CpcE/F裂合酶 |
| ISSN号 | 1000-3096 |
| DOI | DOI: 10.11759/hykx20160512001 |
| 其他题名 | Biosynthesis of unnatural phycobiliproteins using universal bilin lyase CpcE/F |
| 产权排序 | (1) 鲁东大学资源与环境工程学院 ; (2) 中国科学院烟台海岸带研究所 ; (3) 国家海洋局第一海洋研究所 |
| 文献子类 | Article |
| 英文摘要 | Phycobilin lyase enzymes can attach phcobilins to their cognate apoproteins, which play an important role in the biosynthesis of phycobiliproteins. To verify the universal function of lyase cpcE/F, two recombinant plasmids: pCDFDuet-apcA-cpcE/F-hox1-pebS and pCDFDuet-pecA-cpcE/F-hox1-pebS were constructed using genetic engineering methods, and then two unnatural phycobiliproteins, ApcA-PEB and PecA-PEB, were successfully expressed in Escherichia coli, which indicated that cpcE/F could catalyze not only the ligation of PCB with different apoproteins, but also that of PEB and different apoproteins, and thus are universal bilin lyases. The absorption and fluorescence spectrum analysis showed that both unnatural proteins exhibit similar characteristic peaks to those of natural phycobiliproteins with some peaks of isomers and wavelength shifts, and their spectroscopic characteristics were mainly determined by the bilin type carried on them. Furthermore, different apoproteins could also regulate the absorption spectrum and show important influences on the photostability of phycobilins. Our work will provide important information to better understand the biosynthetic mechanism of the phycocybiliproteins and production of unnatural phycobiliproteins with a better fluorescence quality. |
| WOS关键词 | Genetics & Heredity |
| WOS研究方向 | Genetics & Heredity ; 遗传与遗传 |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6016028 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24289]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.中国科学院烟台海岸带可持续发展研究所 2.中国石油大学(华东)生物工程中心; |
| 推荐引用方式 GB/T 7714 | 吝晓君,王祥法,葛保胜,等. 利用多功能裂合酶CpcE/F合成非天然重组藻胆蛋白[J]. 海洋科学,2017,41(3):68-74. |
| APA | 吝晓君,王祥法,葛保胜,&秦松.(2017).利用多功能裂合酶CpcE/F合成非天然重组藻胆蛋白.海洋科学,41(3),68-74. |
| MLA | 吝晓君,et al."利用多功能裂合酶CpcE/F合成非天然重组藻胆蛋白".海洋科学 41.3(2017):68-74. |
入库方式： OAI收割
来源：烟台海岸带研究所
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