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雨生红球藻PHOT的基因克隆和生物信息学分析

文献类型：期刊论文

作者崔红利; 陈军; 王林萍; 胡章立; 秦松
刊名西南农业学报
出版日期2018-01-11
卷号30期号:12页码:2639-2647
关键词Phototropin Full-length of cDNA Structure domain Phylogeny and evolution Haematococcus pluvialis 向光素 cDNA全长 结构域 系统进化 雨生红球藻
ISSN号1001-4829
DOIDOI:10.16213/j.cnki.scjas.2017.12.005
其他题名Gene Cloning and Bioinformatics Analysis of Novel Blue Photoreceptor Phototropin from Green Alga Haematococcus pluvialis
产权排序(1)大连大学生命科学与技术学院 ; (2)中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室 ; (3)中国科学院大学 ; 深圳大学生命与海洋科学学院
文献子类Article
英文摘要【Objective 】In this study,a full-length complementary DNA ( cDNA) sequence of a novel blue photoreceptor phototropin (PHOT),designated Haephot, was cloned, and the bioinformatics from the green alga Haematococcus pluvialis was analyzed.【Method】 The homology cloning and rapid-amplification of cDNA ends ( RACE) strategies were applied to obtain the sequence of Haephot, and its sequence was analyzed.【Result】The cloned cDNA of Haephot contained an open reading frame (ORF) of 2139 bp encoding a protein of 712 amino acids, and the deduced protein had a calculated molecular mass of 78.73 kD with an estimated isoelectric point (pI) of 8.66. Multiple sequence alignment analysis revealed that HaePHOT was homologs to known PHOTs, e. g. , Arabidopsis thaliana (58 % ) and Chlamydomonas reinhardtii (68 % ). Conserved domains analysis showed that the typical catalytic motifs of the PHOT were highly conserved in the protein sequences of HaePHOT. The phylogenetic analysis of the PHOTs implied that HaePHOT had higher similarity to the PHOTs from high plants and green algae, which indicated that they share a common ancestor origin.【Conclusion】The first identified algal phot gene from green alga Haematococcus pluvialis was obtained,which facilitated the expression and functional investigations of PHOT-mediated signaling pathways involved in astaxanthin biosynthesis regulation.
WOS关键词Agriculture
WOS研究方向Agriculture ; 农业
语种中文
CSCD记录号CSCD:6141740
源URL[http://ir.yic.ac.cn/handle/133337/24303]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.大连大学生命科学与技术学院;
2.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用重点实验室;
3.中国科学院大学;
4.深圳大学生命与海洋科学学院
推荐引用方式
GB/T 7714		 崔红利,陈军,王林萍,等. 雨生红球藻PHOT的基因克隆和生物信息学分析[J]. 西南农业学报,2018,30(12):2639-2647.
APA 崔红利,陈军,王林萍,胡章立,&秦松.(2018).雨生红球藻PHOT的基因克隆和生物信息学分析.西南农业学报,30(12),2639-2647.
MLA 崔红利,et al."雨生红球藻PHOT的基因克隆和生物信息学分析".西南农业学报 30.12(2018):2639-2647.

入库方式： OAI收割

来源：烟台海岸带研究所

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