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Necessity of electrically conductive pili for methanogenesis with magnetite stimulation

文献类型：期刊论文

作者Wang, Oumei; Zheng, Shiling; Wang, Bingchen; Wang, Wenjing; Liu, Fanghua
刊名PEERJ
出版日期2018-03-21
卷号6页码:e4541
关键词Geobacter metallireducens Methanosarcina barkeri Electrically conductive pili (e-pili) Direct interspecies electron transfer (DIET) Magnetite Ferrous iron Ethanol metabolism Stimulation Co-cultures Methane
ISSN号2167-8359
DOI10.7717/peerj.4541
产权排序[Wang, Oumei] Binzhou Med Univ, Yantai, Shandong, Peoples R China ; [Zheng, Shiling ; Wang, Bingchen ; Wang, Wenjing ; Liu, Fanghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Shandong, Peoples R China ; [Wang, Bingchen] Univ Chinese Acad Sci, Beijing, Peoples R China
文献子类Article
英文摘要Background: Magnetite-mediated direct interspecies electron transfer (DIET) between Geobacter and Methanosarcina species is increasingly being invoked to explain magnetite stimulation of methane production in anaerobic soils and sediments. Although magnetite-mediated DIET has been documented in defined co-cultures reducing fumarate or nitrate as the electron acceptor, the effects of magnetite have only been inferred in methanogenic systems. Methods: Concentrations of methane and organic acid were analysed with a gas chromatograph and high-performance liquid chromatography, respectively. The concentration of HCl-extractable Fe(II) was determined by the ferrozine method. The association of the defined co-cultures of G. metallireducens and M. barkeri with magnetite was observed with transmission electron micrographs. Results: Magnetite stimulated ethanol metabolism and methane production in defined co-cultures of G. metallireducens and M. barkeri; however, magnetite did not promote methane production in co-cultures initiated with a culture of G. metallireducens that could not produce electrically conductive pili (e-pili), unlike the conductive carbon materials that facilitate DIET in the absence of e-pili. Transmission electron microscopy revealed that G. metallireducens and M. barkeri were closely associated when magnetite was present, as previously observed in G. metallireducens/G. sulfurreducens co-cultures. These results show that magnetite can promote DIET between Geobacter and Methanosarcina species, but not as a substitute for e-pili, and probably functions to facilitate electron transfer from the e-pili to Methanosarcina. Conclusion: In summary, the e-pili are necessary for the stimulation of not only G. metallireducens/G. sulfurreducens, but also methanogenic G. metallireducens/M. barkeri co-cultures with magnetite.
WOS关键词INTERSPECIES ELECTRON-TRANSFER ; C-TYPE CYTOCHROME ; GEOBACTER-METALLIREDUCENS ; PADDY SOIL ; OXIDE REDUCTION ; FE(III) OXIDE ; SP-NOV ; EXCHANGE ; METHANE ; SULFURREDUCENS
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000428693200003
资助机构Major Research Plan of the National Natural Science Foundation of China [91751112] ; General Programme of the National Natural Science Foundation of China [41573071, 41371257] ; Key Research Project of Frontier Science of the Chinese Academy of Sciences [QYZDJ-SSW-DQC015] ; Natural Science Foundation of Shandong Province [ZR2016DQ12] ; Young Taishan Scholars Programme [tsqn20161054]
源URL[http://ir.yic.ac.cn/handle/133337/24383]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Shandong, Peoples R China;
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Binzhou Med Univ, Yantai, Shandong, Peoples R China;
推荐引用方式
GB/T 7714		 Wang, Oumei,Zheng, Shiling,Wang, Bingchen,et al. Necessity of electrically conductive pili for methanogenesis with magnetite stimulation[J]. PEERJ,2018,6:e4541.
APA Wang, Oumei,Zheng, Shiling,Wang, Bingchen,Wang, Wenjing,&Liu, Fanghua.(2018).Necessity of electrically conductive pili for methanogenesis with magnetite stimulation.PEERJ,6,e4541.
MLA Wang, Oumei,et al."Necessity of electrically conductive pili for methanogenesis with magnetite stimulation".PEERJ 6(2018):e4541.

入库方式： OAI收割

来源：烟台海岸带研究所

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