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A cheZ-Like Gene in Azorhizobium caulinodans Is a Key Gene in the Control of Chemotaxis and Colonization of the Host Plant

文献类型：期刊论文

作者Liu, Xiaolin; Liu, Wei; Sun, Yu; Xia, Chunlei; Elmerich, Claudine; Xie, Zhihong
刊名APPLIED AND ENVIRONMENTAL MICROBIOLOGY
出版日期2018-02
卷号84期号:3页码:UNSP e01827-17
关键词Azorhizobium caulinodans CheZ Sesbania rostrata chemotaxis colonization
ISSN号0099-2240
DOI10.1128/AEM.01827-17
产权排序[Liu, Xiaolin ; Liu, Wei ; Sun, Yu ; Xie, Zhihong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China ; [Liu, Xiaolin ; Sun, Yu] Univ Chinese Acad Sci, Beijing, Peoples R China ; [Xia, Chunlei] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol Shandong, Yantai, Peoples R China ; [Elmerich, Claudine] Inst Pasteur, Paris, France
文献子类Article
英文摘要Chemotaxis can provide bacteria with competitive advantages for survival in complex environments. The CheZ chemotaxis protein is a phosphatase, affecting the flagellar motor in Escherichia coli by dephosphorylating the response regulator phosphorylated CheY protein (CheY similar to P) responsible for clockwise rotation. A cheZ gene has been found in Azorhizobium caulinodans ORS571, in contrast to other rhizobial species studied so far. The CheZ protein in strain ORS571 has a conserved motif similar to that corresponding to the phosphatase active site in E. coli. The construction of a cheZ deletion mutant strain and of cheZ mutant strains carrying a mutation in residues of the putative phosphatase active site showed that strain ORS571 participates in chemotaxis and motility, causing a hyperreversal behavior. In addition, the properties of the cheZ deletion mutant revealed that ORS571 CheZ is involved in other physiological processes, since it displayed increased flocculation, biofilm formation, exopolysaccharide (EPS) production, and host root colonization. In particular, it was observed that the expression of several exp genes, involved in EPS synthesis, was upregulated in the cheZ mutant compared to that in the wild type, suggesting that CheZ negatively controls exp gene expression through an unknown mechanism. It is proposed that CheZ influences the Azorhizobium-plant association by negatively regulating early colonization via the regulation of EPS production. This report established that CheZ in A. caulinodans plays roles in chemotaxis and the symbiotic association with the host plant. IMPORTANCE Chemotaxis allows bacteria to swim toward plant roots and is beneficial to the establishment of various plant-microbe associations. The level of CheY phosphorylation (CheY similar to P) is central to the chemotaxis signal transduction. The mechanism of the signal termination of CheY similar to P remains poorly characterized among Alphaproteobacteria, except for Sinorhizobium meliloti, which does not contain CheZ but which controls CheY similar to P dephosphorylation through a phosphate sink mechanism. Azorhizobium caulinodans ORS571, a microsymbiont of Sesbania rostrata, has an orphan cheZ gene besides two cheY genes similar to those in S. meliloti. In addition to controlling the chemotaxis response, the CheZ-like protein in strain ORS571 is playing a role by decreasing bacterial adhesion to the host plant, in contrast to the general situation where chemotaxis-associated proteins promote adhesion. In this study, we identified a CheZ-like protein among Alphaproteobacteria functioning in chemotaxis and the A. caulinodans-S. rostrata symbiosis.
WOS关键词GRAM-NEGATIVE BACTERIA ; SINORHIZOBIUM-MELILOTI ; SESBANIA-ROSTRATA ; ESCHERICHIA-COLI ; AZOSPIRILLUM-BRASILENSE ; RHIZOBIUM-MELILOTI ; BIOFILM FORMATION ; CELL-AGGREGATION ; SIGNAL-TRANSDUCTION ; RESPONSE REGULATOR
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
语种英语
WOS记录号WOS:000423770000008
资助机构National Natural Science Foundation of China [31370108, 31570063] ; One Hundred-Talent Plan of the Chinese Academy of Sciences ; High-Tech Industrialization Cooperation Funds of Jilin province ; Chinese Academy of Sciences [2017SYHZ0007] ; Shandong Key Research and Development Program [2016CYJS05A01-1] ; Institut Pasteur, Paris, France
源URL[http://ir.yic.ac.cn/handle/133337/24401]  
专题烟台海岸带研究所_中科院海岸带环境过程与生态修复重点实验室
烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol Shandong, Yantai, Peoples R China;
2.Inst Pasteur, Paris, France
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai, Peoples R China;
4.Univ Chinese Acad Sci, Beijing, Peoples R China;
推荐引用方式
GB/T 7714		 Liu, Xiaolin,Liu, Wei,Sun, Yu,et al. A cheZ-Like Gene in Azorhizobium caulinodans Is a Key Gene in the Control of Chemotaxis and Colonization of the Host Plant[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2018,84(3):UNSP e01827-17.
APA Liu, Xiaolin,Liu, Wei,Sun, Yu,Xia, Chunlei,Elmerich, Claudine,&Xie, Zhihong.(2018).A cheZ-Like Gene in Azorhizobium caulinodans Is a Key Gene in the Control of Chemotaxis and Colonization of the Host Plant.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,84(3),UNSP e01827-17.
MLA Liu, Xiaolin,et al."A cheZ-Like Gene in Azorhizobium caulinodans Is a Key Gene in the Control of Chemotaxis and Colonization of the Host Plant".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 84.3(2018):UNSP e01827-17.

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来源：烟台海岸带研究所

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