Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli
文献类型：期刊论文
| 作者 | Ge, Baosheng; Chen, Yao; Yu, Qian; Lin, Xiaojun; Li, Jiqiang; Qin, Song
|
| 刊名 | PROCESS BIOCHEMISTRY
 |
| 出版日期 | 2018-08 |
| 卷号 | 71页码:23-30 |
| ISSN号 | 1359-5113 |
| 关键词 | Phycocyanobilin Heme biosynthetic pathway 5-Aminolevulinic acid Regulation Combinatorial biosynthesis Escherichia coli |
| DOI | 10.1016/j.procbio.2018.05.011 |
| 产权排序 | [Ge, Baosheng ; Chen, Yao ; Yu, Qian ; Lin, Xiaojun ; Li, Jiqiang] China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China ; [Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Due to its biochemical and spectroscopic properties, phycocyanobilin (PCB) is of special interest for a variety of applications, but the production of PCB from Spirulina is complicated and costly. Here we report the assembly and regulation of the entire heme biosynthetic pathway for combinatorial biosynthesis of PCB in Escherichia coli. Our results shows that a plasmid containing bicistronic expression of hoxi and pcyA yields 6.64 mg/L PCB, which is higher than that of plasmid with one promoter for both genes (p < 0.01). Up-regulation of hemB, hemG, hemH, hemA(s), and hemL individually or simultaneously does not significantly improve the yield of PCB, but induces accumulation of 5-Aminolevulinic acid (ALA) and heme. Increasing the copy number of hoxi and pcyA genes slightly improves the production of PCB by enhancing the expression level of Hox1 and PcyA enzymes, suggesting that this two-step enzymatic reaction is the rate-limiting step for the production of PCB. This work provides new important insights into the regulatory mechanism of the heme biosynthesis pathway for production of valuable chemicals. |
| WOS关键词 | 5-AMINOLEVULINIC ACID ; BILE-PIGMENTS ; ALLOPHYCOCYANIN ; TETRAPYRROLES ; CHROMOPHORE ; DISORDERS ; SPIRULINA ; EFFICIENT ; GLUCOSE ; PROTEIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical |
| 语种 | 英语 |
| WOS记录号 | WOS:000442004200004 |
| 资助机构 | National key Research and Development Plan of China [2016YFE0106700] ; National Natural Science Foundation of China [21373271, 21673294] ; Key Research Project of Shandong Province [2017GGX40114] ; Open Fund of Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China [OF2015N009] ; Fundamental Research Funds for the Central Universities |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24506]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China 2.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ge, Baosheng,Chen, Yao,Yu, Qian,et al. Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli[J]. PROCESS BIOCHEMISTRY,2018,71:23-30. |
| APA | Ge, Baosheng,Chen, Yao,Yu, Qian,Lin, Xiaojun,Li, Jiqiang,&Qin, Song.(2018).Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli.PROCESS BIOCHEMISTRY,71,23-30. |
| MLA | Ge, Baosheng,et al."Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli".PROCESS BIOCHEMISTRY 71(2018):23-30. |
入库方式： OAI收割
来源：烟台海岸带研究所
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