中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Extraction and characterization of type I collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering

文献类型:期刊论文

作者Li, Jie; Wang, Mingchao; Qiao, Yingyun; Tian, Yuanyu; Liu, Junhong; Qin, Song; Wu, Wenhui
刊名PROCESS BIOCHEMISTRY
出版日期2018-11
卷号74页码:156-163
关键词Tilapia Collagen Fish skin Marine biomaterials Microfiber matrix scaffolds
ISSN号1359-5113
DOI10.1016/j.procbio.2018.07.009
产权排序[Li, Jie ; Wang, Mingchao ; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264000, Peoples R China ; [Qiao, Yingyun ; Tian, Yuanyu] China Univ Petr East China, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China ; [Liu, Junhong] Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266000, Peoples R China ; [Wu, Wenhui] Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Marine Pharmacol, Shanghai 201306, Peoples R China
文献子类Article
英文摘要Aquatic origin collagen (Aqua-collagen) has been pursued as an alternative to mammalian origins. Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were successfully extracted from the skin of tilapia with the yield about 19-20% (basis of lyophilized dry weight), and examined for their physico-functional and structural properties. Both ASC and PSC containing at, a2 chains were characterized to be type I collagen and had lower denaturation temperature compared to mammalian origins. PSC (low telopeptides collagen) was selected for biomedical scaffolds construction due to its low immunogenicity. SEM analysis of fish collagen scaffolds showed a wide range of pore size distribution, high porosity, and high surface area-to-volume ratios. The tilapia collagen microfiber matrix scaffolds were grafted beneath the dorsal skin in 96 mice towards tissue regeneration, with bovine collagen microfiber matrix scaffolds (Avitene" UltraFoamn" sponge) serving as control. Biocompatibility evaluation in the dorsal tissue showed that implanted scaffolds degraded completely after 20 days with no pathological inflammatory tissue responses. These findings indicated that aqua-collagens microfiber matrix scaffolds were highly biocompatible in nature, exploring its feasibility for the development of scaffolds in tissue engineering.
WOS关键词PEPSIN-SOLUBLE COLLAGEN ; EXTRACELLULAR-MATRIX ; CONNECTIVE TISSUES ; CROSS-LINKING ; OUTER SKIN ; BONE ; ACID ; BIOMATERIALS ; SCALE ; DENATURATION
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Engineering, Chemical
语种英语
WOS记录号WOS:000447577800020
资助机构key program of the Chinese Academy of Sciences [KFZD-SW-218] ; Science and Technology Development Project of Shandong Province [2015GSF115032] ; 2016 Strategic Emerging Industry Development Fund (Starting Fund for Marine Economy Development and Innovation Demonstration City) [YHCX-SW-Y-201701]
源URL[http://ir.yic.ac.cn/handle/133337/24561]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264000, Peoples R China;
2.China Univ Petr East China, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China;
3.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266000, Peoples R China;
4.Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Marine Pharmacol, Shanghai 201306, Peoples R China
推荐引用方式
GB/T 7714
Li, Jie,Wang, Mingchao,Qiao, Yingyun,et al. Extraction and characterization of type I collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering[J]. PROCESS BIOCHEMISTRY,2018,74:156-163.
APA Li, Jie.,Wang, Mingchao.,Qiao, Yingyun.,Tian, Yuanyu.,Liu, Junhong.,...&Wu, Wenhui.(2018).Extraction and characterization of type I collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering.PROCESS BIOCHEMISTRY,74,156-163.
MLA Li, Jie,et al."Extraction and characterization of type I collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering".PROCESS BIOCHEMISTRY 74(2018):156-163.

入库方式: OAI收割

来源:烟台海岸带研究所

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