Gateway技术构建菊芋块茎全长cDNA文库及EST测序分析
文献类型：期刊论文
| 作者 | 杨少丽 ; 秦松
|
| 刊名 | 生命科学研究
 |
| 出版日期 | 2018-10-31 |
| 卷号 | 22期号:05页码:370-374+383 |
| 关键词 | 菊芋 cDNA文库 Gateway技术 表达序列标签(EST) 基因注释 Jerusalem artichoke cDNA library Gateway technology expressed sequence tag (EST) gene annotation |
| ISSN号 | 1007-7847 |
| DOI | 10.16605/j.cnki.1007-7847.2018.05.004 |
| 其他题名 | Construction of a cDNA Library Using Gateway Technology and Analysis of Expressed Sequence Tags (ESTs) from Jerusalem Artichoke |
| 产权排序 | (1)中国科学院烟台海岸带研究所 ; (2) |
| 英文摘要 | A full-length cDNA library was constructed from the tuber of Jerusalem artichoke by recombinating the full-length cDNAs with the Gateway donor vector of pDONR222 for gene discovery and functional characterization. The cDNA library quality analysis showed that the capacity of original library was 5.76×106 CFU. The inserted fragments were mainly in the range of 1~3 000 bp and the recombination rate was 100% (24/24). These results indicated that the cDNA library had a high quality. EST sequencing was conducted and 2 639 high-quality ESTs were obtained, and 1 895 unigenes were obtained after assembly. NCBI database (NR) homologous alignment analysis showed that a total of 1 533 unigenes (80.9%) had significant homology with known genes. GO classification showed that the proportion of binding and catalytic activity were highest among the molecular function taxon. The cDNA library can be well used for functional genomics, novel gene screening, high-through EST sequencing and the preparation of cDNA microarray of Jerusalem artichoke. |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6357445 |
| 资助机构 | 烟台市科技计划项目#2015ZH067 ; 国家自然科学基金项目#41001336 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24664]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 中国科学院烟台海岸带研究所; |
| 推荐引用方式 GB/T 7714 | 杨少丽,秦松. Gateway技术构建菊芋块茎全长cDNA文库及EST测序分析[J]. 生命科学研究,2018,22(05):370-374+383. |
| APA | 杨少丽,&秦松.(2018).Gateway技术构建菊芋块茎全长cDNA文库及EST测序分析.生命科学研究,22(05),370-374+383. |
| MLA | 杨少丽,et al."Gateway技术构建菊芋块茎全长cDNA文库及EST测序分析".生命科学研究 22.05(2018):370-374+383. |
入库方式： OAI收割
来源：烟台海岸带研究所
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