褐藻表面可培养褐藻酸降解菌的原位筛选
文献类型：期刊论文
| 作者 | 王明鹏; 王学江; 陈蕾 |
| 刊名 | 微生物学通报
 |
| 出版日期 | 2018-06-20 |
| 卷号 | 45期号:09页码:1853-1860 |
| 关键词 | 褐藻酸降解 菌种筛选 铜藻 革兰氏碘液 |
| ISSN号 | 0253-2654 |
| DOI | 10.13344/j.microbiol.china.180152 |
| 其他题名 | In situ screening of cultivable alginate-degrading microorganism on surface of brown seaweed |
| 产权排序 | (1)五洲丰农业科技有限公司 ; (2)中国科学院烟台海岸带研究所 |
| 英文摘要 | [Background] Alginate oligosaccharides produced by enzymatic hydrolysis of alginate have diverse biological functions such as anti-oxidation, anti-tumor, induction of immune regulation, and regulation of plant growth, and exhibit broad application prospects in the fields of food and medicine. Mass screening of alginate-degrading bacteria is beneficial to obtain new structures and functions of alginate oligosaccharides, which is conducive to actively promoting the industrial progress of oligosaccharides. [Objective] High-efficiency screening was carried out for alginate-degrading strains and exploring the in situ microbial resources of seaweeds with development prospects. [Methods] The only carbon source of sodium alginate was used to select the microorganisms on the surface of brown seaweed. The chromogenic reaction of gram iodine solution indicated the characteristics of microorganisms to degrade alginate. The activity of alginate lyase was determined by Oxford cup method. [Results] A total of 81 colonies were obtained from the surface of the algae samples. Twenty-eight strains were screened by clear circle staining. Seven strains of alginate-degrading bacteria were identified by 16S rRNA gene sequencing analysis. They belonged to the genus Bacillus (3/7), Arthrobacter (1/7), Desemzia (1/7), Brachybacterium (1/7) and Streptosporangium (1/7). The genera Arthrobacter, Desemzia, Brachybacterium and Streptosporangium have not been previously reported to produce alginate lyase. Further analysis showed that T-1 strain (Bacillus) had maximum enzyme production and highest enzyme activity. [Conclusion] Seven alginate lyase-excreting strains were isolated, and the enzyme activities of the strains were compared by Gram's iodine coloration combined with the Oxford cup method. This indicates that the screening method is simple, efficient and suitable for large-scale screening of alginate lyase-excreting microorganisms. |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6327329 |
| 资助机构 | 国家自然科学基金青年基金项目#41401285 ; 烟台市重点研发计划#2016YTZH072 ; 哈尔滨工业大学城市水资源与水环境国家重点实验室开放基金项目#HC201713 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24696]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.五洲丰农业科技有限公司; 2.中国科学院烟台海岸带研究所 |
| 推荐引用方式 GB/T 7714 | 王明鹏,王学江,陈蕾. 褐藻表面可培养褐藻酸降解菌的原位筛选[J]. 微生物学通报,2018,45(09):1853-1860. |
| APA | 王明鹏,王学江,&陈蕾.(2018).褐藻表面可培养褐藻酸降解菌的原位筛选.微生物学通报,45(09),1853-1860. |
| MLA | 王明鹏,et al."褐藻表面可培养褐藻酸降解菌的原位筛选".微生物学通报 45.09(2018):1853-1860. |
入库方式： OAI收割
来源：烟台海岸带研究所
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