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重组LHCⅡ与叶绿素a的体外结合及其光电性能表征

文献类型：期刊论文

作者侯琪琪; 于倩; 李泉; 李荣贵; 秦松; 葛保胜
刊名生物学杂志
出版日期2018-03-30
卷号35期号:04页码:34-38
关键词LHCⅡ 叶绿素 染料敏化太阳能电池
ISSN号2095-1736
DOI10.3969/j.issn.2095-1736.2018.04.034
其他题名In vitro reconstitution of LHCⅡ recombinant with chlorophyll-a and characterization of its photoelectric properties
产权排序(1)青岛大学生命科学学院 ; (2)中国石油大学(华东)生物工程与技术中心 ; (3)中国科学院烟台海岸带研究所
英文摘要The lhcⅡ gene from Chlamydomonas reinhardtii was cloned and expressed in Escherichia coli. The LHCⅡrecombinant was prepared using genetic engineering technology,and then reconstructed with chlorophyll a in vitro. The saturation curve of the complex of LHCⅡ/chlorophyll-a was obtained. The free chlorophyll a and the complexs of LHCⅡ/chlorophyll a (at same concentration) were fixed on the TiO_2 electrode,and the photoelectric conversion properties of these two materials were compared. One recombinant LHCⅡ bound with 8 chlorophyll a molecules. Under the conditions of AM1.5,light intensity of 100 mW/cm~2,shortcut circuit current of LHC Ⅱ/chlorophyll-a complex is 0.493 mA/cm~2,open circuit voltage is 0.491 V,fill-factor is 0.679,and the efficiency is 0.165%. In comparison,shortcut circuit current of free chlorophyll a is 0.596 mA/cm~2,open circuit voltage is 0.482 V,and fill-factor is 0.676, efficiency is 0.194%. The recombinant LHCⅡ protein obtained from E. coli BL21 was successfully combined with chlorophyll a in vitro with similar binding activity as its native proteins. The reconstituted pigment-protein complex fulfilled the light harvesting and electron transport functions in a dye-sensitized solar cell system,and showed promising photoelectric properties at lower concentrations. In this study,large-scale preparation of LHCⅡ,the binding activity of recombinant protein LHCⅡ with chlorophyll in vitro,and its application in photoelectric conversion has laid a foundation.
语种中文
CSCD记录号CSCD:6310437
资助机构国家重点研发计划项目#2016YFE0106700 ; 国家自然科学基金项目#41176144
源URL[http://ir.yic.ac.cn/handle/133337/24712]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.中国科学院烟台海岸带研究所
2.青岛大学生命科学学院;
3.中国石油大学(华东)生物工程与技术中心;
推荐引用方式
GB/T 7714		 侯琪琪,于倩,李泉,等. 重组LHCⅡ与叶绿素a的体外结合及其光电性能表征[J]. 生物学杂志,2018,35(04):34-38.
APA 侯琪琪,于倩,李泉,李荣贵,秦松,&葛保胜.(2018).重组LHCⅡ与叶绿素a的体外结合及其光电性能表征.生物学杂志,35(04),34-38.
MLA 侯琪琪,et al."重组LHCⅡ与叶绿素a的体外结合及其光电性能表征".生物学杂志 35.04(2018):34-38.

入库方式： OAI收割

来源：烟台海岸带研究所

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