钝顶螺旋藻C-藻蓝蛋白的能量传递动力学
文献类型：期刊论文
| 作者 | 王肖肖; 李文军 ; 谢明远; 赵福利; 杨革; 刘琪; 秦松
|
| 刊名 | 科学通报
 |
| 出版日期 | 2018-03-10 |
| 卷号 | 63期号:07页码:641-648 |
| 关键词 | C-藻蓝蛋白六聚体 时间分辨光谱 能量传递 |
| ISSN号 | 0023-074X |
| DOI | 10.1360/N972017-01026 |
| 其他题名 | The energy transfer dynamics in C-phycocyanin hexamer from Spirulina platensis |
| 产权排序 | (1)曲阜师范大学生命科学学院 ; (2)中国科学院烟台海岸带研究所 ; (3)中山大学物理学院 ; (4)烟台大学药学院 |
| 英文摘要 | Photosynthesis is an ancient and important conversion of solar light to biological energy in photosynthetic organisms. Phycobilisome (PBS) system in cyanobacteria and red algae is one of two major light-harvesting systems of photosynthetic oxygen organisms. PBS is aggregation of water-soluble phycobiliproteins (PBPs) which are distinctively colored group of disk-shaped macromolecular proteins bearing covalently attached open-chain tetrapyrroles known as phycobilins. PBPs are capable to absorb solar light and transfer energy to the chlorophyll of photosynthetic reaction center with a high efficiency. Light energy absorbed by phycobilins is transmitted between the subunits, and then transmitted between different PBPs, finally to the photoreaction center located on the thylakoid membrane. The energy transfer between PBPs is very fast and high efficiency. Because energy can be transferred in different chromophores and there are a large number of chromophores in PBPs, the energy transfer path of PBPs is complex and diverse. In the past 20 years, energy transfer process of PBPs have been analyzed by time-resolved fluorescence spectroscopy because a large shift of the fluorescence peaks can be clearly observed. For example, Zhang et al. estimated the time constant for energy transfer from PC (rod) to APC (core) as 18 ps and 55 ps based on PBPs crystal structures and time-resolved fluorescence spectra of the rod-core complex. However, due to the technical bottleneck of theoretical simulation and spectral experimental detection, the research on the physical mechanism of photosynthetic energy transfer is relatively slow. In recent years, the ultrafast spectrum time resolution has been continuously improved, which provides the conditions for studying the energy transfer of PBPs. In this paper, the mechanism of energy transfer in C-phycocyanin hexamer from Spirulina platensis was studied by ultrafast time-resolved fluorescence spectroscopy. The energy transfer pathway and the related transfer parameters in the C-phycocyanin hexamer were identified by the fitting of the fluorescence decay curve at different detection wavelengths. It was proved that the energy transfer of C-phycocyanin hexamer had four time components from the experiment, which are 6, 22, 280 and 1470 ps, respectively, then the time components were identified and discussed. This study reflects the energy transfer kinetics between different chromophores in C-phycocyanin and provides important experimental data for solving the relationship between the structure and function of PBPs. At the same time, our study has important value for the understanding of the primary process of photosynthesis. In the future, if the resolution of a streak camera can be raised to femtoseconds, it is possible to find more energy transfer paths, and thus it is expected to make a breakthrough in the ultrafast and ultra-efficient energy transfer mechanism of PBPs. |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6213819 |
| 资助机构 | 国家重点研发项目#2016YFB0601001 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24719]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.中国科学院烟台海岸带研究所; 2.曲阜师范大学生命科学学院; 3.中山大学物理学院; 4.烟台大学药学院 |
| 推荐引用方式 GB/T 7714 | 王肖肖,李文军,谢明远,等. 钝顶螺旋藻C-藻蓝蛋白的能量传递动力学[J]. 科学通报,2018,63(07):641-648. |
| APA | 王肖肖.,李文军.,谢明远.,赵福利.,杨革.,...&秦松.(2018).钝顶螺旋藻C-藻蓝蛋白的能量传递动力学.科学通报,63(07),641-648. |
| MLA | 王肖肖,et al."钝顶螺旋藻C-藻蓝蛋白的能量传递动力学".科学通报 63.07(2018):641-648. |
入库方式： OAI收割
来源：烟台海岸带研究所
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