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异化铁还原梭菌Clostridium bifermentans EZ-1产氢与电化学特性

文献类型：期刊论文

作者张月超; 肖雷雷; 王欧美; 刘芳华
刊名微生物学报
出版日期2018-02-08
卷号58期号:04页码:525-537
ISSN号0001-6209
关键词滨海湿地 双酶梭菌 产电 产氢 异化铁还原
DOI10.13343/j.cnki.wsxb.20170504
其他题名Hydrogen-producing and electrochemical properties of a dissimilatory Fe(III) reducer Clostridium bifermentans EZ-1
产权排序(1)中国科学院烟台海岸带研究所海岸带生物学与生物资源利用所重点实验室 ; (2)中国科学院大学 ; (3)滨州医学院
英文摘要[Objective] To obtain and characterize a versatile strain isolated from the soil of Yellow River Delta.[Methods] The strain was isolated by anaerobic technologies and identified by sequencing the 16S rRNA gene.The morphology was depicted with Gram staining and scanning electron microscope.High performance liquid chromatography and gas chromatography were used to analyze the metabolic substrates and productions.Ferric citrate (FeC6H5O7),ferrihydrite (FeOOH) and magnetite (Fe_3O_4) were supplied to detect the capability of Fe(Ⅲ) reduction of this strain.Microbial fuel cells were constructed with graphite as electrodes to test the electrochemical activity of this strain.[Results] Compared to Clostridium bifermentans,the similarity of 16S rRNA gene is 97.99%.It is a rod,Gram-positive bacterium,which can use glucose to produce hydrogen,carbon dioxide,acetate and butyrate.The Fe(Ⅲ) reduction results showed that it could reduce soluble ferric citrate,amorphous ferrihydrite and crystal magnetite.Furthermore,the electrochemical activity was detected and the maximum current density peaked at 6.5 mA/m~2.[Conclusion] In this work,a versatile strain which is capable of producing hydrogen,electricity and reducing iron oxide,was successfully isolated from the soil of Yellow River Delta named as Clostridium bifermentans EZ-1.
语种中文
CSCD记录号CSCD:6214299
资助机构国家自然科学基金#41573071,国家自然科学基金#41371257 ; 国家自然科学基金#91751112 ; 国家自然科学基金#41703075 ; 中国科学院前沿科学重点研究项目#QYZDJ-SSWDQC015 ; 山东省自然科学杰出青年基金#ZR2016DQ12 ; “泰山学者”青年专家计划#tsqn20161054
源URL[http://ir.yic.ac.cn/handle/133337/24726]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
作者单位1.滨州医学院
2.中国科学院烟台海岸带研究所海岸带生物学与生物资源利用所重点实验室;
3.中国科学院大学;
推荐引用方式
GB/T 7714		 张月超,肖雷雷,王欧美,等. 异化铁还原梭菌Clostridium bifermentans EZ-1产氢与电化学特性[J]. 微生物学报,2018,58(04):525-537.
APA 张月超,肖雷雷,王欧美,&刘芳华.(2018).异化铁还原梭菌Clostridium bifermentans EZ-1产氢与电化学特性.微生物学报,58(04),525-537.
MLA 张月超,et al."异化铁还原梭菌Clostridium bifermentans EZ-1产氢与电化学特性".微生物学报 58.04(2018):525-537.

入库方式： OAI收割

来源：烟台海岸带研究所

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