Reductive degradation of chloramphenicol by Geobacter metallireducens
文献类型：期刊论文
| 作者 | Xu, HD; Xiao, LL ; Zheng, SL; Zhang, YC; Li, JJ; Liu, FH
|
| 刊名 | SCIENCE CHINA-TECHNOLOGICAL SCIENCES
 |
| 出版日期 | 2019-10 |
| 卷号 | 62期号:10页码:1688-1694 |
| 关键词 | chloramphenicol Geobacter metallireducens dechlorination extracellular electron transfer reduction |
| ISSN号 | 1674-7321 |
| DOI | 10.1007/s11431-018-9415-2 |
| 产权排序 | [Xu HengDuo] Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China ; [Xu HengDuo ; Xiao LeiLei ; Zheng ShiLing ; Zhang YueChao ; Li JiaJia ; Liu FangHua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China ; [Zhang YueChao ; Li JiaJia ; Liu FangHua] Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China ; [Zhang YueChao ; Li JiaJia] Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Geobacter metallireducens is known to be capable of removing nitroaromatic compounds via an oxidation mode. However, little attention has been paid to investigate the reductive removal of chlorinated nitroaromatic compounds by G. metallireducens. In this study, G. metallireducens was used to reduce chloramphenicol (CAP), a typical chlorinated nitroaromatic antibiotic. Cyclic voltammograms and chronoamperometry highlighted a higher peak current for CAP reduction by G. metallireducens compared to the control without bacteria. G. metallireducens efficiently reduced CAP (20 mg/L) with acetate as the sole electron donor, and the removal efficiency reached (97.6 +/- 4.9)% within 6 d. Aromatic amine (AMCl2), AMCl (dechlorinated AMCl2) and AM (dechlorinated AMCl) were identified as reduction products by liquid chromatography-mass spectrometry. However, the removal efficiency declined to (25.0 +/- 3.6)% when the CAP dosage increased to 80 mg/L. Transcriptomic analysis indicated the significant upregulation of genes related to electron transfer, such as pilus assembly protein gene (2.8 folds), NADH-quinone oxidoreductase subunit K2 gene (4.5 folds) and many c-type cytochrome genes such as cytochrome c biogenesis protein ResB (Gmet 2901, 4.6 folds), cytochrome c (Gmet 0335, 4.4 folds) and cytochrome c7 (Gmet 2902, 3.4 folds). Furthermore, a gene related to chlorinated contaminant removal (Gmet 1046, 5.4 folds) was also upregulated, possibly resulting in enhanced CAP reduction. This work deepened our knowledge of the bioremediation ability of G. metallireducens with respect to environmental contaminants and provided a potential strategy to treat antibiotics with electrochemically active bacteria. |
| WOS关键词 | CARBON ; PHARMACEUTICALS ; FLORFENICOL ; RESISTANCE ; MECHANISM ; OXIDATION ; ELECTRODE ; REMOVAL ; BIOFILM ; METAL |
| WOS研究方向 | Engineering, Multidisciplinary ; Materials Science, Multidisciplinary |
| 语种 | 英语 |
| WOS记录号 | WOS:000504097800004 |
| 资助机构 | Postdoctoral Science Foundation of ChinaChina Postdoctoral Science Foundation [2018M632735] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [91751112, 41573071] ; Senior User Project of RV KEXUE [KEXUE2018G01] ; Natural Science FoundationNational Natural Science Foundation of China [JQ201608] ; Young Taishan Scholars Program of Shandong Province [tsqn20161054] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24838]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao 266071, Shandong, Peoples R China; 2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Utilizat, Yantai 264003, Peoples R China; 3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, HD,Xiao, LL,Zheng, SL,et al. Reductive degradation of chloramphenicol by Geobacter metallireducens[J]. SCIENCE CHINA-TECHNOLOGICAL SCIENCES,2019,62(10):1688-1694. |
| APA | Xu, HD,Xiao, LL,Zheng, SL,Zhang, YC,Li, JJ,&Liu, FH.(2019).Reductive degradation of chloramphenicol by Geobacter metallireducens.SCIENCE CHINA-TECHNOLOGICAL SCIENCES,62(10),1688-1694. |
| MLA | Xu, HD,et al."Reductive degradation of chloramphenicol by Geobacter metallireducens".SCIENCE CHINA-TECHNOLOGICAL SCIENCES 62.10(2019):1688-1694. |
入库方式： OAI收割
来源：烟台海岸带研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。