Biosynthesis of phycocyanobilin in recombinant Escherichia coli
文献类型：期刊论文
| 作者 | Ma, CB; Li, WJ ; Ge, BS; Lin, J; Qin, S
|
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2020-03 |
| 卷号 | 38期号:2页码:529-538 |
| 关键词 | combinational biosynthesis phycocyanobilin response surface methodology antioxidant |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-019-9060-6 |
| 产权排序 | [Ma, Chengbo ; Lin, Jian] Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China ; [Li, Wenjun ; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China ; [Ge, Baosheng] China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China ; [Li, Wenjun ; Qin, Song] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | The recombinant expression of phycocyanobilin (PCB) was carried out in Escherichia coli, and the best fermentation conditions of recombinant E. coli biosynthesized PCB are optimized in the response surface methodology to improve PCB production. The recombinant PCB is extracted, isolated, and purified by methanol and chloroform extraction. Recombinant PCB is validated in UV-vis spectroscopy, high-pressure liquid chromatography, and mass spectrometry. In addition, the anti-oxidant activities of the recombinant PCB are determined. The best induction conditions that optimized by Design Expert 8.0 software include: lactose concentration 4 mmol/L, induction temperature 24.69 degrees C, induction time 4.6 h, and induction duration 13.57 h, under which the PCB expression level reached approximately 13 mg PCB/L, which is more than four times of previously reported 3 mg PCB/L. The maximum absorption peak of the recombinant PCB is located at 680 nm with a high fluorescence intensity of 470 nm. The recombinant PCB has a good ability to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radicals. |
| WOS关键词 | SP PCC 6803 ; C-PHYCOCYANIN ; ANTIOXIDANT ; CHROMOPHORES ; BILIPROTEINS ; EXPRESSION |
| WOS研究方向 | Limnology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:000522834800019 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24855]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China; 2.Yantai Univ, Coll Life Sci, Yantai 264005, Peoples R China; 3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China 4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ma, CB,Li, WJ,Ge, BS,et al. Biosynthesis of phycocyanobilin in recombinant Escherichia coli[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2020,38(2):529-538. |
| APA | Ma, CB,Li, WJ,Ge, BS,Lin, J,&Qin, S.(2020).Biosynthesis of phycocyanobilin in recombinant Escherichia coli.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,38(2),529-538. |
| MLA | Ma, CB,et al."Biosynthesis of phycocyanobilin in recombinant Escherichia coli".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 38.2(2020):529-538. |
入库方式： OAI收割
来源：烟台海岸带研究所
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