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Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide

文献类型：期刊论文

作者Li, WJ; Jiang, TT; Pu, Y; Jiao, XD; Tan, WQ; Qin, S
刊名MICRO & NANO LETTERS
出版日期2019-03-06
卷号14期号:3页码:344-348
关键词biochemistry biosensors nanosensors nanofabrication fluorescence molecular biophysics radiation quenching chemical sensors sugar proteins biomedical optical imaging graphene compounds GO-CS low concentrations competitive binding maltose-binding protein recombinant phycocyanin far-red fluorescence MBP-rPC rPC emission rPC fluorescence linear detection range glucose biosensor fluorescence quenching chitosan-modified graphene oxide low-molecular-weight chitosan glucose detection fluorescence resonance energy transfer graphene oxide nanomaterial mass 0 1 mg to 1 0 mg CO
ISSN号1750-0443
DOI10.1049/mnl.2018.5269
产权排序[Li, Wenjun ; Jiao, Xudong ; Tan, Wenqiang ; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China ; [Jiang, Tingting] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China ; [Pu, Yang] Ludong Univ, Sch Agr, Yantai 264025, Peoples R China
文献子类Article
英文摘要A novel graphene oxide (GO) nanomaterial, which was rapidly and simply synthesised and conjugated with low-molecular-weight chitosan (CS), was used to develop a simple, sensitive GO biosensor system for glucose detection by fluorescence resonance energy transfer. The GO-CS was used to detect low concentrations of glucose based on competitive binding with maltose-binding protein (MBP). The -subunit of recombinant phycocyanin (rPC), which emits far-red fluorescence, was used to label MBP (MBP-rPC). The rPC emission was quenched by binding between GO-CS and MBP. However, in the presence of glucose, GO-CS was out-competed for binding to MBP, leading to rPC fluorescence. Glucose was sensitively and selectively detected with this biosensor, with a linear detection range of 0.1-1 mg glucose/ml. The limit of detection for glucose was approximate to 0.05 mg/ml.
WOS关键词FRET BIOSENSOR ; PURIFICATION ; MANAGEMENT ; PROTEIN ; SENSOR ; DNA
WOS研究方向Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
语种英语
WOS记录号WOS:000461572800025
资助机构Marine Economy Innovation Development Demonstration Project State Oceanic Administration of China [YHCX-SW-P-201701, YHCX-SW-Y-201701] ; Scientific Research Foundation of Ludong University [ly2014041]
源URL[http://ir.yic.ac.cn/handle/133337/24977]  
专题烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室
海岸带生物资源高效利用研究与发展中心
作者单位1.Ludong Univ, Sch Agr, Yantai 264025, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
3.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China;
推荐引用方式
GB/T 7714		 Li, WJ,Jiang, TT,Pu, Y,et al. Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide[J]. MICRO & NANO LETTERS,2019,14(3):344-348.
APA Li, WJ,Jiang, TT,Pu, Y,Jiao, XD,Tan, WQ,&Qin, S.(2019).Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide.MICRO & NANO LETTERS,14(3),344-348.
MLA Li, WJ,et al."Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide".MICRO & NANO LETTERS 14.3(2019):344-348.

入库方式： OAI收割

来源：烟台海岸带研究所

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