Transient expression of the enhanced green fluorescent protein (egfp) gene in Sargassum horneri
文献类型：期刊论文
| 作者 | Pang, YL; Li, Y; Liu, ZY ; Cui, YL; Qin, S
|
| 刊名 | JOURNAL OF OCEANOLOGY AND LIMNOLOGY
 |
| 出版日期 | 2019-03 |
| 卷号 | 37期号:2页码:651-656 |
| 关键词 | green fluorescent protein (GFP) particle bombardment Sargassum horneri transgenesis |
| ISSN号 | 2096-5508 |
| DOI | 10.1007/s00343-019-8014-3 |
| 产权排序 | [Pang Yunlong ; Liu Zhengyi ; Cui Yulin ; Qin Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China ; [Pang Yunlong] Univ Chinese Acad Sci, Beijing 101408, Peoples R China ; [Li Yan] State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China |
| 文献子类 | Article |
| 英文摘要 | Sargassum horneri is a macroalga widespread in North Asia-Pacific region, and these years its bloom has caused huge damage to the environment and the economic in China. To make up the blank on genetic engineering research, a transient transformation system for the multicellular marine brown alga S. horneri was established in this research. The algae used in this research were collected from the Yellow Sea of China and verified as a same species S. horneri with analysis of molecular markers. The S. horneri parietal leaves were transformed with the enhanced green fluorescent gene as the reporter by micro-particle bombardment. The results show that GFP is an effective transgene reporter for S. horneri and that particle bombardment is a suitable method for transformation of S. horneri. Through selection of four different promoters for EGFP and six groups' bombardment characters, the highest transformation efficiency approximately 1.31% was got with the vector pEGFP-N1 at bombardment characters 900 spi and 6 cm distance. This research paves a way for the further research and application of S. horneri. |
| WOS关键词 | TRANSFORMATION ; CHLOROPHYTA ; PLASMIDS |
| WOS研究方向 | Limnology ; Oceanography |
| 语种 | 英语 |
| WOS记录号 | WOS:000464895600023 |
| 资助机构 | National Key Research and Development Program of China [2016YFC1402106] ; Project of Innovation & Development of Marine Economy [HHCL201803] ; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41406192, 41376139] ; Science and Technology Service Network Initiative of Chinese Academy of Sciences [KFJ-STS-ZDTP-023] |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24982]  |
| 专题 | 烟台海岸带研究所_海岸带生物学与生物资源利用所重点实验室 |
| 作者单位 | 1.State Ocean Adm, Yantai Ocean Environm Monitoring Cent Stn, Yantai 264006, Peoples R China 2.Univ Chinese Acad Sci, Beijing 101408, Peoples R China; 3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Pang, YL,Li, Y,Liu, ZY,et al. Transient expression of the enhanced green fluorescent protein (egfp) gene in Sargassum horneri[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2019,37(2):651-656. |
| APA | Pang, YL,Li, Y,Liu, ZY,Cui, YL,&Qin, S.(2019).Transient expression of the enhanced green fluorescent protein (egfp) gene in Sargassum horneri.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,37(2),651-656. |
| MLA | Pang, YL,et al."Transient expression of the enhanced green fluorescent protein (egfp) gene in Sargassum horneri".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 37.2(2019):651-656. |
入库方式： OAI收割
来源：烟台海岸带研究所
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