海蜇幼体环介导等温扩增快速检测方法的建立和应用
文献类型：期刊论文
| 作者 | 孙婷婷; 刘中援; 梁丽琨; 董志军 |
| 刊名 | 应用海洋学学报
 |
| 出版日期 | 2017-11-15 |
| 卷号 | 36期号:4页码:538-544 |
| 关键词 | marine biology Rhopilema esculentum loop-mediated isothermal amplification method rapid detection polyps 海洋生物学 海蜇 环介导等温扩增 快速检测 螅状幼体 |
| ISSN号 | 2095-4972 |
| DOI | DOI:10.3969/J.ISSN.2095-4972.2017.04.010 |
| 其他题名 | Development a rapid assay to detect the edible jellyfish Rhopilema esculentum larvae using loop-mediated isothermal amplification method |
| 产权排序 | (1) 烟台大学生命科学学院 ; (2) 中国科学院烟台海岸带研究所 |
| 文献子类 | Article |
| 英文摘要 | It is difficult to identify the scyphomedusae larvae by traditional morphological methods,due to the complex morphological characteristics in different stages of their life cycle. For early identifying and detecting of this species,a simple and effective method using the loop-mediated isothermal amplification method (LAMP) has been developed. LAMP primers of Rhopilema esculentum were designed based on the mitochondrial cytochrome c oxidase subunit Ⅰ (COⅠ) gene. The amplification conditions,including the betaine and dNTPs concentrations,the inner primer to outer primer concentration ratio,reaction time and temperature,were optimized. The specificity,sensitivity and repeatability of the method were also tested. Our results show that the primers had specificity and repeatability. Samples from different geographic populations of R. esculentum and polyps produced positive results,while the other non-target scyphomedusae species produced negative results. In addition,the LAMP assay was more sensitive than conventional PCR. The detection limit of LAMP method was 0. 1 ng DNA. Therefore,the established LAMP assay is a sensitive,specific,fast,and easily performed method for detection of the edible jellyfish R. esculentum. |
| WOS关键词 | Oceanography |
| WOS研究方向 | Oceanography ; 海洋学 |
| 语种 | 中文 |
| CSCD记录号 | CSCD:6118554 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/24274]  |
| 专题 | 海岸带生物资源高效利用研究与发展中心 |
| 作者单位 | 1.中国科学院烟台海岸带研究所 2.烟台大学生命科学学院; |
| 推荐引用方式 GB/T 7714 | 孙婷婷,刘中援,梁丽琨,等. 海蜇幼体环介导等温扩增快速检测方法的建立和应用[J]. 应用海洋学学报,2017,36(4):538-544. |
| APA | 孙婷婷,刘中援,梁丽琨,&董志军.(2017).海蜇幼体环介导等温扩增快速检测方法的建立和应用.应用海洋学学报,36(4),538-544. |
| MLA | 孙婷婷,et al."海蜇幼体环介导等温扩增快速检测方法的建立和应用".应用海洋学学报 36.4(2017):538-544. |
入库方式： OAI收割
来源：烟台海岸带研究所
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