Improved suppression of circulating complement does not block acute vascular rejection of pig-to-rhesus monkey cardiac transplants
文献类型：期刊论文
| 作者 | Chen G2; Sun QY1; Wang XM2; Shen SQ2; Guo H2; Wang H2; Wu Y2; Wang WY1; Xiong YL1; Chen S*2 |
| 刊名 | XENOTRANSPLANTATION
 |
| 出版日期 | 2004 |
| 卷号 | 11期号:2页码:123-132 |
| 关键词 | Acute Vascular Rejection Heart Transplantation Pig-to-rhesus Monkey Model Xenotransplantaion Yunnan-cobra Venom Factor |
| ISSN号 | 0908-665X |
| 英文摘要 | At present, acute vascular rejection (AVR) remains a primary obstacle inhibiting long-term graft survival in the pig-to-non-human primate transplant model. The present study was undertaken to determine whether repetitive injection of low dose Yunnan-cobra venom factor (Y-CVF), a potent complement inhibitor derived from the venom of Naja kaouthia can completely abrogate hemolytic complement activity and subsequently improve the results in a pig-to-rhesus monkey heterotopic heart transplant model. Nine adult rhesus monkeys received a heterotopic heart transplant from wild-type pigs and the recipients were allocated into two groups: group 1 (n = 4) received repetitive injection of low dose Y-CVF until the end of the study and group 2 (n = 5) did not receive Y-CVF. All recipients were treated with cyclosporine A (CsA), cyclophosphamide (CyP) and steroids. Repetitive Y-CVF treatment led to very dramatic fall in CH50 and serum C3 levels (CH50 < 3 units/C3 remained undetectable throughout the experiment) and successfully prevented hyperacute rejection (HAR), while three of five animals in group 2 underwent HAR. However, the continuous suppression of circulating complement did not prevent AVR and the grafts in group 1 survived from 8 to 13 days. Despite undetectable C3 in circulating blood, C3 deposition was present in these grafts. The venular thrombosis was the predominant histopathologic feature of AVR. We conclude that repetitive injection of low dose Y-CVF can be used to continuously suppress circulating complement in a very potent manner and successfully prevent HAR. However, this therapy did not inhibit complement deposition in the graft and failed to prevent AVR. These data suggest that using alternative pig donors [i.e. human decay accelerating factor (hDAF)-transgenic] in combination with the systemic use of complement inhibitors may be necessary to further control complement activation and improve survival in pig-to-non-human primate xenotransplant model. |
| URL标识 | 查看原文 |
| 资助机构 | This study was supported by a grant ‘‘National Natural Science Foundation of China (39993434)’’ and by ‘‘the National High Technology Research and Development Program (863 program) of China (101-05-05-01)’’. ; This study was supported by a grant ‘‘National Natural Science Foundation of China (39993434)’’ and by ‘‘the National High Technology Research and Development Program (863 program) of China (101-05-05-01)’’. ; This study was supported by a grant ‘‘National Natural Science Foundation of China (39993434)’’ and by ‘‘the National High Technology Research and Development Program (863 program) of China (101-05-05-01)’’. ; This study was supported by a grant ‘‘National Natural Science Foundation of China (39993434)’’ and by ‘‘the National High Technology Research and Development Program (863 program) of China (101-05-05-01)’’. |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5325]  |
| 专题 | 昆明动物研究所_其他 |
| 通讯作者 | schen@tjh.tjmu.edu.cn |
| 作者单位 | 1.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China |
| 推荐引用方式 GB/T 7714 | Chen G,Sun QY,Wang XM,et al. Improved suppression of circulating complement does not block acute vascular rejection of pig-to-rhesus monkey cardiac transplants[J]. XENOTRANSPLANTATION,2004,11(2):123-132. |
| APA | Chen G.,Sun QY.,Wang XM.,Shen SQ.,Guo H.,...&schen@tjh.tjmu.edu.cn.(2004).Improved suppression of circulating complement does not block acute vascular rejection of pig-to-rhesus monkey cardiac transplants.XENOTRANSPLANTATION,11(2),123-132. |
| MLA | Chen G,et al."Improved suppression of circulating complement does not block acute vascular rejection of pig-to-rhesus monkey cardiac transplants".XENOTRANSPLANTATION 11.2(2004):123-132. |
入库方式： OAI收割
来源：昆明动物研究所
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