TMVA, a novel GPIb-binding protein, significantly prevents platelet microthrombi formation and prolongs discordant cardiac xenograft survival
文献类型：期刊论文
| 作者 | Chen G1; Wei Q2; Wang XM1; Wang WY2; Xiong YL2; Chen S*1; schen@tjh.tjmu.edu.cn |
| 刊名 | XENOTRANSPLANTATION
 |
| 出版日期 | 2004 |
| 卷号 | 11期号:2页码:203-209 |
| 关键词 | Glycoprotein (Gp) Ib Platelet Aggregation Tmva Trimeresurus Mucrosquamatus Xenotransplantation |
| ISSN号 | 0908-665X |
| 英文摘要 | In xenotransplantation, donor endothelium is the first target of immunological attack. Activation of the endothelial cell by preformed natural antibodies leads to platelet binding via the interaction of the glycoprotein (GP) Ib and von Willebrand factor (vWF). TMVA is a novel GPIb-binding protein purified from the venom of Trimeresurus mucrosquamatus. In this study, the inhibitory effect of TMVA on platelet aggregation in rats and the effect on discordant guinea pig-to-rat cardiac xenograft survival were investigated. Three doses (8, 20 or 40 mug/kg) of TMVA were infused intravenously to 30 rats respectively. Platelet aggregation rate was assayed 0.5, 12, and 24 h after TMVA administration. Wister rats underwent guinea pig cardiac cervical heterotopic transplantation using single dosing of TMVA (20 mug/kg, i.v., 0.5 h before reperfusion). Additionally, levels of TXB2 and 6-keto-PGF(1alpha) within rejected graft tissues were determined by radioimmunoassay. Treatment with TMVA at a dose of 20 or 40 mug/kg resulted in complete inhibition of platelet aggregation 0.5 h after TMVA administration. Rats receiving guinea pig cardiac xenografts after TMVA therapy had significantly prolonged xenograft survival. Histologic and immunopathologic analysis of cardiac xenografts in TMVA treatment group showed no intragraft platelet microthrombi formation and fibrin deposition. Additionally, the ratio of 6-keto-PGF(1alpha) to TXB2 in TMVA treatment group was significantly higher than those in control group. We conclude that the use of this novel GPIb-binding protein was very effective in preventing platelet microthrombi formation and fibrin deposition in a guinea pig-to-rat model and resulted in prolongation of xenograft survival. The increased ratio of PGI(2)/TXA(2) in TMVA treatment group may protect xenografts from the endothelial cell activation and contribute to the prolongation of xenograft survival. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 资助机构 | This study was supported by a grant of ‘National Natural Science Foundation of China (39993434)’ and by ‘the National High Technology Research and Development Program (863 program) of China (101-05-05-01)’. ; This study was supported by a grant of ‘National Natural Science Foundation of China (39993434)’ and by ‘the National High Technology Research and Development Program (863 program) of China (101-05-05-01)’. ; This study was supported by a grant of ‘National Natural Science Foundation of China (39993434)’ and by ‘the National High Technology Research and Development Program (863 program) of China (101-05-05-01)’. ; This study was supported by a grant of ‘National Natural Science Foundation of China (39993434)’ and by ‘the National High Technology Research and Development Program (863 program) of China (101-05-05-01)’. |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5327]  |
| 专题 | 昆明动物研究所_其他 |
| 通讯作者 | schen@tjh.tjmu.edu.cn |
| 作者单位 | 1.Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China 2.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China |
| 推荐引用方式 GB/T 7714 | Chen G,Wei Q,Wang XM,et al. TMVA, a novel GPIb-binding protein, significantly prevents platelet microthrombi formation and prolongs discordant cardiac xenograft survival[J]. XENOTRANSPLANTATION,2004,11(2):203-209. |
| APA | Chen G.,Wei Q.,Wang XM.,Wang WY.,Xiong YL.,...&schen@tjh.tjmu.edu.cn.(2004).TMVA, a novel GPIb-binding protein, significantly prevents platelet microthrombi formation and prolongs discordant cardiac xenograft survival.XENOTRANSPLANTATION,11(2),203-209. |
| MLA | Chen G,et al."TMVA, a novel GPIb-binding protein, significantly prevents platelet microthrombi formation and prolongs discordant cardiac xenograft survival".XENOTRANSPLANTATION 11.2(2004):203-209. |
入库方式： OAI收割
来源：昆明动物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。