Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains
文献类型：期刊论文
| 作者 | Zhu LL1,3; Yuan C1,3; Chen Z1,3; Wang WY*2,3; Huang MD*1,3; wangwy@mail.kiz.ac.cn; mhuang@fjirsm.ac.cn |
| 刊名 | TOXICON
 |
| 出版日期 | 2010 |
| 卷号 | 55期号:37655页码:375-380 |
| 关键词 | Jerdonitin Recombinant Protein Expression Snake Venom Metalloproteinases |
| ISSN号 | 0041-0101 |
| 英文摘要 | Jerdonitin is a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains. In this study, we established a high-level expression system in Pichia pastoris and developed a purification strategy for the recombinant Jerdonitin. This recombinant Jerdonitin degraded fibrinogen at a level of activity comparable with its wild type. The effects of recombinant Jerdonitin on inhibiting ADP-induced human platelet aggregation were in a dose-dependent manner with an IC50 of 248 nM. In addition, we reported here that Jerdonitin can significantly inhibit the growth of several cell lines, including human liver cancer cells (Bel7402), human leukemia cells (K562) and human gastric carcinoma cells (BGC823). This study offers recombinant Jerdonitin that will be valuable for further functional and structural studies of Jerdonitin. |
| URL标识 | 查看原文 |
| 资助机构 | Our research was supported by grants from CAS (KSCX2- YW-R-082), Fujian NSF (2009I0029), NSFC (30625011, 30811130467, 30770429) and MOST (2007CB914304 and 2006AA02A313). ; Our research was supported by grants from CAS (KSCX2- YW-R-082), Fujian NSF (2009I0029), NSFC (30625011, 30811130467, 30770429) and MOST (2007CB914304 and 2006AA02A313). ; Our research was supported by grants from CAS (KSCX2- YW-R-082), Fujian NSF (2009I0029), NSFC (30625011, 30811130467, 30770429) and MOST (2007CB914304 and 2006AA02A313). ; Our research was supported by grants from CAS (KSCX2- YW-R-082), Fujian NSF (2009I0029), NSFC (30625011, 30811130467, 30770429) and MOST (2007CB914304 and 2006AA02A313). |
| 公开日期 | 2010-08-24 |
| 源URL | [http://159.226.149.42:8088/handle/152453/5395]  |
| 专题 | 昆明动物研究所_其他 |
| 通讯作者 | wangwy@mail.kiz.ac.cn; mhuang@fjirsm.ac.cn |
| 作者单位 | 1.State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, 155 Yang Qiao Xi Lu, Fuzhou, Fujian 350002, China 2.Department of Animal Toxinology, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 3.Graduate School of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 10039, China |
| 推荐引用方式 GB/T 7714 | Zhu LL,Yuan C,Chen Z,et al. Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains[J]. TOXICON,2010,55(37655):375-380. |
| APA | Zhu LL.,Yuan C.,Chen Z.,Wang WY*.,Huang MD*.,...&mhuang@fjirsm.ac.cn.(2010).Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains.TOXICON,55(37655),375-380. |
| MLA | Zhu LL,et al."Expression, purification and characterization of recombinant Jerdonitin, a P-II class snake venom metalloproteinase comprising metalloproteinase and disintegrin domains".TOXICON 55.37655(2010):375-380. |
入库方式： OAI收割
来源：昆明动物研究所
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