中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 成都山地灾害与环境研究所
  3. 中国科学院水利部成都山地灾害与环境研究所
Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity

文献类型：期刊论文

作者Khadka Sujan2,6,8; Adhikari Sanjib8; Thapa Alina3,6; Panday Raju4; Adhikari Manjila4; Sapkota Sanjeep5,6,8; Regmi Ramesh Sharma8; Adhikari Namita Paudel3,6; Proshad Ram1,6; Koirala Niranjan7
刊名CURRENT MICROBIOLOGY
出版日期2020
页码DOI: 10.1007/s00284-020-01976-7
ISSN号0343-8651
DOI10.1007/s00284-020-01976-7
通讯作者Adhikari, Sanjib(sanadh26@gmail.com)
产权排序2
文献子类Article;Early Access
英文摘要The current study was designed to isolate, identify and characterize a Bacillus sp. capable of producing protease and exhibiting antifungal activity. A highly potent bacterium capable of producing protease abundantly was isolated from the soil collected from the waste pit near Microbiology Laboratory of Birendra Multiple Campus, Bharatpur and later on identified as Lysinibacillus fusiformis strain SK on the basis of morphological, physiological, biochemical and 16S rDNA gene sequencing techniques. The strain SK showed 98.36% similarity with L. fusiformis strain NBRC 15717. Using R-programming statistical analysis tool, the optimum incubation time for the highest average protease production (APP) (47.2 U/mL) was found to be 22 h at 50 degrees C and both incubation time and temperature showed a significant impact on the production of protease (P < 0.01). The maximum average relative protease activity (ARPA) was observed at pH 7.8 and 48 degrees C, whereas the least ARPA was observed in the presence of 80 g/L NaCl and 10 g/L HgCl2 (P < 0.01). The newly isolated bacterial strain also exhibited strong antifungal activity against aflatoxigenic Aspergillus flavus and Aspergillus parasiticus suggesting that it can be a potential candidate for protease production and activity over a wider range of temperature and pH as well as for synthesizing effective antifungal compounds.
电子版国际标准刊号1432-0991
WOS关键词ASPERGILLUS-FLAVUS GENOMICS ; ALKALINE PROTEASE ; SP NOV. ; BACILLUS ; ITURIN ; OCHRATOXIN ; MYCOTOXINS
WOS研究方向Microbiology
语种英语
出版者SPRINGER
WOS记录号WOS:000523426600002
源URL[http://ir.imde.ac.cn/handle/131551/34116]  
专题中国科学院水利部成都山地灾害与环境研究所
通讯作者Adhikari Sanjib
作者单位1.Key Laboratory of Mountain Surface Process and Ecological Regulation, Institute of Mountain Hazards and Environment, Chinese Academy of Sciences, Chengdu 610041, China;
2.State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
3.State Key Laboratory of Alpine Ecology and Biodiversity, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing 100101, China;
4.Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China;
5.State Key Laboratory of Respiratory Disease, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou 510530, China;
6.University of Chinese Academy of Sciences, Beijing 100049, China8)Department of Natural Products Research, Dr. Koirala Research Institute for Biotechnology and Biodiversity, Kathmandu 44613, Nepal;
7.Faculty of Science and Technology, University of Macau, Macau SAR 999078, China
8.Department of Microbiology, Birendra Multiple Campus, Tribhuvan University, Chitwan, Bharatpur 44200, Nepal;
推荐引用方式
GB/T 7714		 Khadka Sujan,Adhikari Sanjib,Thapa Alina,et al. Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity[J]. CURRENT MICROBIOLOGY,2020:DOI: 10.1007/s00284-020-01976-7.
APA Khadka Sujan.,Adhikari Sanjib.,Thapa Alina.,Panday Raju.,Adhikari Manjila.,...&Koirala Niranjan.(2020).Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity.CURRENT MICROBIOLOGY,DOI: 10.1007/s00284-020-01976-7.
MLA Khadka Sujan,et al."Screening and Optimization of Newly Isolated Thermotolerant Lysinibacillus fusiformis Strain SK for Protease and Antifungal Activity".CURRENT MICROBIOLOGY (2020):DOI: 10.1007/s00284-020-01976-7.

入库方式： OAI收割

来源：成都山地灾害与环境研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。