中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 地质与地球物理研究所
  3. 中国科学院地质与地球物理研究所
  4. 中国科学院地球与行星物理重点实验室
Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1

文献类型：期刊论文

作者Yu, Jiacheng2,3,4; Zhang, Tongwei3,4; Xu, Huangtao2,3,4; Dong, Xiaoli1; Cai, Yao3,4; Pan, Yongxin2,3,4; Cao, Changqian2,3,4
刊名RSC ADVANCES
出版日期2019-12-01
卷号9期号:67页码:39381-39393
DOI10.1039/c9ra07397c
英文摘要Thermostable nanoparticles have numerous applications in catalysis and in the oil/gas industry. However, synthesizing these nanoparticles requires expensive polymers. Here, a novel thermostable ferritin named PcFn, originally from the hyperthermophilic archaeon Pyrococcus yayanosii CH1, was overexpressed in Escherichia coli, purified and characterized, which could successfully direct the synthesis of thermostable magnetoferritins (M-PcFn) with monodispersed iron oxide nanoparticles in one step. Transmission electron microscopy and magnetic measurements show that the cores of the M-PcFn have an average diameter of 4.7 nm, are well-crystalline and superparamagnetic. Both the PcFn and M-PcFn can resist temperatures up to 110 degrees C, which is significantly higher than for human H-chain ferritin (HFn) and M-HFn, and comparable to temperatures previously reported for Pyrococcus furiosus ferritin (PfFn) and M-PfFn. After heating at 110 degrees C for 30 minutes, PcFn and M-PcFn maintained their secondary structures and PcFn retained 87.4% of its iron uptake activity. This remarkable thermostability of PcFn and M-PcFn suggests potential applications in elevated temperature environments.
WOS关键词AMINO-ACID PROPERTIES ; THERMAL-STABILITY ; PROTEIN CAGE ; TEMPERATURE ; MAGNETOFERRITIN ; STABILIZATION ; NANOCLUSTERS ; UNIFORM ; BRINE ; SIZE
资助项目National Natural Science Foundation of China (NSFC)[41574062] ; National Natural Science Foundation of China (NSFC)[41774076] ; National Natural Science Foundation of China (NSFC)[41621004] ; Key Program of Chinese Academy of Sciences[QYZDJ-SSW-DQC024]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000501820700048
出版者ROYAL SOC CHEMISTRY
资助机构National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences ; National Natural Science Foundation of China (NSFC) ; National Natural Science Foundation of China (NSFC) ; Key Program of Chinese Academy of Sciences ; Key Program of Chinese Academy of Sciences
源URL[http://ir.iggcas.ac.cn/handle/132A11/95840]  
专题地质与地球物理研究所_中国科学院地球与行星物理重点实验室
通讯作者Cao, Changqian
作者单位1.Univ Calgary, Dept Geosci, Calgary, AB T2N 1N4, Canada
2.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Phys Inst Geol & Geophys, Key Lab Earth & Planetary Phys, Biogeomagnetism Grp,Paleomagnetism & Geochronol L, Beijing 100029, Peoples R China
4.Chinese Acad Sci, Innovat Acad Earth Sci, Beijing 100029, Peoples R China
推荐引用方式
GB/T 7714		 Yu, Jiacheng,Zhang, Tongwei,Xu, Huangtao,et al. Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1[J]. RSC ADVANCES,2019,9(67):39381-39393.
APA Yu, Jiacheng.,Zhang, Tongwei.,Xu, Huangtao.,Dong, Xiaoli.,Cai, Yao.,...&Cao, Changqian.(2019).Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1.RSC ADVANCES,9(67),39381-39393.
MLA Yu, Jiacheng,et al."Thermostable iron oxide nanoparticle synthesis within recombinant ferritins from the hyperthermophile Pyrococcus yayanosii CH1".RSC ADVANCES 9.67(2019):39381-39393.

入库方式： OAI收割

来源：地质与地球物理研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。