Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification
文献类型：期刊论文
| 作者 | Ullah, Saif2; Irfan, Muhammad1; Sajjad, Wasim3; Rana, Qurrat Ul Ain2; Hasan, Fariha2; Khan, Samiullah2; Badshah, Malik2; Shah, Aamer Ali2 |
| 刊名 | FOOD BIOTECHNOLOGY
 |
| 出版日期 | 2019-10-02 |
| 卷号 | 33期号:4页码:353-372 |
| 关键词 | Bacillus pumilus xylanase size exclusion chromatography SDS-PAGE organic solvents |
| ISSN号 | 0890-5436 |
| DOI | 10.1080/08905436.2019.1674157 |
| 英文摘要 | A xylanase-producing bacterium Bacillus pumilus K22 was isolated from hot spring located in Manghopir area, Karachi, Pakistan. The enzyme was purified to homogeneity by acetone precipitation and size exclusion chromatography. The molecular weight was determined to be approximately 24 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The purified xylanase retained its activity at wide range of temperature (20-70?C) and pH (5.0?10.0) with maximum stability at 50?C and pH 8.0. Xylanase activity was inhibited by Zn2+, Cu2+, and Hg2+ metal ions while it remained stable in the presence of Mn2+, Ca2+ and Mg2+. The activity was also inhibited by chemical reagents such as EDTA, PMSF, and ?-mercaptoethanol. The enzyme was stable in the presence of all organic solvents (20%) except formaldehyde and glycerol, which inhibited its activity. Treatment of tomato pulp with purified xylanase-increased juice yield (30%), clarity (9%), and reducing sugars (69%), indicating an improvement in physico-chemical characteristics of the tomato pulp. |
| WOS关键词 | CELLULASE-FREE XYLANASE ; ENZYMATIC CLARIFICATION ; MICROBIAL XYLANASES ; SAPODILLA JUICE ; PURIFICATION ; STRAIN ; OPTIMIZATION ; ENZYMES ; CLONING |
| 资助项目 | Higher Education Commission of Pakistan |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Food Science & Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000494491700005 |
| 出版者 | TAYLOR & FRANCIS INC |
| 资助机构 | Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan ; Higher Education Commission of Pakistan |
| 源URL | [http://ir.iggcas.ac.cn/handle/132A11/95735]  |
| 专题 | 地质与地球物理研究所_兰州油气中心 |
| 通讯作者 | Shah, Aamer Ali |
| 作者单位 | 1.Sarhad Univ Sci & Informat Technol, Inst Biol Sci, Peshawar, Pakistan 2.Quaid I Azam Univ, Fac Biol Sci, Dept Microbiol, Islamabad 45320, Pakistan 3.Chinese Acad Sci, Inst Geol & Geophys, Gansu Prov Key Lab Petr Resources Res, Key Lab Petr Resources, Lanzhou, Gansu, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ullah, Saif,Irfan, Muhammad,Sajjad, Wasim,et al. Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification[J]. FOOD BIOTECHNOLOGY,2019,33(4):353-372. |
| APA | Ullah, Saif.,Irfan, Muhammad.,Sajjad, Wasim.,Rana, Qurrat Ul Ain.,Hasan, Fariha.,...&Shah, Aamer Ali.(2019).Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification.FOOD BIOTECHNOLOGY,33(4),353-372. |
| MLA | Ullah, Saif,et al."Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification".FOOD BIOTECHNOLOGY 33.4(2019):353-372. |
入库方式： OAI收割
来源：地质与地球物理研究所
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