High-throughput sequencing reveals the gut and lung prokaryotic community profiles of the Chinese giant salamander (Andrias davidianus)
文献类型:期刊论文
作者 | Wu, Zhenbing4,5; Gatesoupe, Francois-Joel3; Zhang, Qianqian2,5; Wang, Xiehao5; Feng, Yuqing4,5; Wang, Shuyi4,5; Feng, Dongyue1; Li, Aihua2,5 |
刊名 | MOLECULAR BIOLOGY REPORTS |
出版日期 | 2019-10-01 |
卷号 | 46期号:5页码:5143-5154 |
ISSN号 | 0301-4851 |
关键词 | Chinese giant salamander (Andrias davidianus) High-throughput sequencing Intestinal tract Lung Prokaryotic community |
DOI | 10.1007/s11033-019-04972-8 |
英文摘要 | Increasing attention has been attracted to host microbiota, due to their vital impact on host health. Little is known about the microbiota of the Chinese giant salamander (Andrias davidianus), in spite of the high economic and scientific value of this endangered species. This study was designed to characterise and compare the gut and lung prokaryotic communities of the Chinese giant salamander by high-throughput sequencing. Our study showed that the giant salamander had a lung prokaryotic community that clustered separately from its intestinal microbiota. Statistical analysis (LEfSe) revealed that the bacterial populations were dominated by Geobacter, Sulfurimonas, and Dechloromonas from Proteobacteria phylum, and Corynebacterium from Actinobacteria phylum in the lung, while Parabacteroides, Bacteroides, and PW3 from Bacteroidetes phylum, and Oscillospira from Firmicutes phylum were predominant in the intestine. A particularly innovative finding was the fairly high abundance of Archaea, especially methanogenic Euryarchaeota. The gut dominant Archaea were Methanocorpusculum and Thermoplasmata vadinCA11, while Methanosaeta and Methanoculleus were the main Archaea in the lung. PICRUSt analysis revealed differentiated functional profiles between the intestinal miacrobiota and the lung microbiota. Specially, some microbial metabolic functions were significantly more active in the intestinal microbiota, while the functional genes involved in infectious diseases were much richer in the lung microbiota. This study characterized the prokaryotic microbial community profiles in the gut and lung of the Chinese giant salamander, providing foundational support for future study seeking to understand microbiota of the giant salamander and the role of its microbiota on infectious diseases. |
WOS关键词 | BACTERIAL COMMUNITIES ; MICROBIOME ; DISEASE ; DIVERSITY ; EVOLUTION ; FISH |
WOS研究方向 | Biochemistry & Molecular Biology |
语种 | 英语 |
出版者 | SPRINGER |
WOS记录号 | WOS:000495590600047 |
源URL | [http://ir.ihb.ac.cn/handle/342005/35371] |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前) |
通讯作者 | Feng, Dongyue; Li, Aihua |
作者单位 | 1.Minist Agr, Natl Fisheries Tech Extens Ctr, Beijing 100125, Peoples R China 2.Huazhong Agr Univ, Freshwater Aquaculture Collaborat Innovat Ctr Hub, Wuhan 430070, Hubei, Peoples R China 3.Ifremer, Ctr Bretagne, Nutr Metab & Aquaculture, INRA, F-29280 Plouzane, France 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Wu, Zhenbing,Gatesoupe, Francois-Joel,Zhang, Qianqian,et al. High-throughput sequencing reveals the gut and lung prokaryotic community profiles of the Chinese giant salamander (Andrias davidianus)[J]. MOLECULAR BIOLOGY REPORTS,2019,46(5):5143-5154. |
APA | Wu, Zhenbing.,Gatesoupe, Francois-Joel.,Zhang, Qianqian.,Wang, Xiehao.,Feng, Yuqing.,...&Li, Aihua.(2019).High-throughput sequencing reveals the gut and lung prokaryotic community profiles of the Chinese giant salamander (Andrias davidianus).MOLECULAR BIOLOGY REPORTS,46(5),5143-5154. |
MLA | Wu, Zhenbing,et al."High-throughput sequencing reveals the gut and lung prokaryotic community profiles of the Chinese giant salamander (Andrias davidianus)".MOLECULAR BIOLOGY REPORTS 46.5(2019):5143-5154. |
入库方式: OAI收割
来源:水生生物研究所
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