Susceptibility and immune responses after challenge with Flavobacterium columnare and Pseudomonas fluorescens in conventional and specific pathogen-free rare minnow (Gobiocypris rarus)
文献类型：期刊论文
| 作者 | Su, Liangxia1,2; Xu, Chunsen1,2; Cai, Lei1,2,3; Qiu, Ning1,2; Hou, Miaomiao2; Wang, Jianwei2 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2020-03-01 |
| 卷号 | 98页码:875-886 |
| 关键词 | Rare minnow Flavobacterium columnare Pseudomonas fluorescens Susceptibility Immune response |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2019.11.047 |
| 通讯作者 | Wang, Jianwei(wangjw@ihb.ac.cn) |
| 英文摘要 | The susceptibility of fish from different culture environments to bacterial infection is not well known. The susceptibility and pathological changes of conventional (CV) and specific pathogen-free (SPF) rare minnow (Gobiocypris rarus) infected with two gram-negative bacteria, Flavobacterium columnare and Pseudomonas fluorescens are investigated. Rare minnows were intraperitoneally challenged with two bacterial species to first determine semi-lethal doses (LD50), and then with the LD50 dose, determine innate immune response. Infected rare minnows developed characteristic red bellies and then died. LD50 doses of F. columnare and P. fluorescens were 4.586 x 10(8) cfu/mL and 2.319 x 10(10) cfu/mL for CV rare minnow, and 2.575 x 10(8) cfu/mL and 1.935 x 10(10) cfu/mL, respectively, for SPF rare minnow. The results of RT-PCR showed that the highest levels of toll-like receptor 3 (TLR3), interleukin-6 (IL-6), interferon-2 (IFN-2) and rare minnow Z-DNA binding protein kinase (GrPKZ) mRNA were noticed at 6-48 h post-infection (hpi). In addition, TLR3, IL-6 and IFN-2 in F. colwnnare challenged rare minnow were more highly expressed than those in P. fluorescens challenged rare minnow, whereas as opposed in the expression of GrPKZ mRNA. Stimulation of innate immune responses is closely related to bacterial virulence. SPF rare minnow might be more susceptible to these bacteria than CV rare minnow, possibly due to their clean environment and lack of resistance. We speculate that clean environment renders rare minnow more susceptible to bacterial infections. |
| WOS关键词 | EXPRESSION ANALYSIS ; MOLECULAR-CLONING ; CHANNEL CATFISH ; TLR3 GENE ; CARP ; DISEASE ; INFECTION ; MODEL ; FISH |
| 资助项目 | Technology R & D Program of Hubei Province, China[2015BCE098] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000516880900092 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 资助机构 | Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China ; Technology R & D Program of Hubei Province, China |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/35955]  |
| 专题 | 水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文 |
| 通讯作者 | Wang, Jianwei |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing, Peoples R China 2.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 3.Guangdong Lab Anim Monitoring Inst, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, Liangxia,Xu, Chunsen,Cai, Lei,et al. Susceptibility and immune responses after challenge with Flavobacterium columnare and Pseudomonas fluorescens in conventional and specific pathogen-free rare minnow (Gobiocypris rarus)[J]. FISH & SHELLFISH IMMUNOLOGY,2020,98:875-886. |
| APA | Su, Liangxia,Xu, Chunsen,Cai, Lei,Qiu, Ning,Hou, Miaomiao,&Wang, Jianwei.(2020).Susceptibility and immune responses after challenge with Flavobacterium columnare and Pseudomonas fluorescens in conventional and specific pathogen-free rare minnow (Gobiocypris rarus).FISH & SHELLFISH IMMUNOLOGY,98,875-886. |
| MLA | Su, Liangxia,et al."Susceptibility and immune responses after challenge with Flavobacterium columnare and Pseudomonas fluorescens in conventional and specific pathogen-free rare minnow (Gobiocypris rarus)".FISH & SHELLFISH IMMUNOLOGY 98(2020):875-886. |
入库方式： OAI收割
来源：水生生物研究所
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