China has over 1320 freshwater fish species, 877 of which are endemic. In recent decades, over-exploitation and landscape pressures have threatened them and led to a severe aquatic biodiversity crisis. In response, large-scale fishing bans have been promulgated to protect freshwater biodiversity in major Chinese rivers since the early 1980s. Here, we present the historical background and current challenges to the fishing bans. Implementing large-scale fishing bans may help improve China's current freshwater biological resources and biodiversity to some extent. But implementing fishing bans alone is not sufficient to solve the crisis because of shortcomings of the current bans and expanding human pressures in most river basins. Thus, we recommend regulating other anthropogenic pressures, expanding duration and extent of current fishing regulations, establishing a comprehensive monitoring program, and initiating basin-scale ecological rehabilitation. These programs are also needed in other developing countries facing similar biodiversity crises and human pressures.