Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate Affects Lipid Metabolism in Zebrafish Larvae via DNA Methylation Modification
文献类型：期刊论文
| 作者 | Guo, Wei1,5; Hang, Jian5; Wu, Shengmin4,5; Shi, Xiongjie3; Wang, Qiangwei2; Zhou, Bingsheng5 |
| 刊名 | ENVIRONMENTAL SCIENCE & TECHNOLOGY
 |
| 出版日期 | 2020-01-07 |
| 卷号 | 54期号:1页码:355-363 |
| ISSN号 | 0013-936X |
| DOI | 10.1021/acs.est.9b05796 |
| 通讯作者 | Zhou, Bingsheng(bszhou@ihb.ac.cn) |
| 英文摘要 | Bis(2-ethylhexyl)-2,3,4,S-tetrabromophthalate (TBPH) is a ubiquitous environmental contaminant, but its toxicity is not fully understood. Accordingly, we investigated the effects of TBPH and its metabolite, mono-(2-ethyhexyl)-tetrabromophthalate (TBMEHP), on lipid metabolism using a zebrafish model. The molecular docking study revealed that TBPH and TBMEHP bind to zebrafish peroxisome proliferator-activated receptor gamma (PPAR gamma), with binding energies similar to rosiglitazone, a PPAR gamma agonist. Zebrafish embryos 0.75 hpf were exposed to TBPH (0.2-2000 nM) or TBMEHP (0.2-2000 nM) until 72 hpf, and their effects on PPAR gamma-mediated lipid metabolism were evaluated. Significant regional DNA demethylation of the PPAR gamma promoter was observed in the larvae at 72 hpf. Demethylation of the PPAR gamma promoter accompanied by upregulation of tet1 and tet2 transcription caused upregulation of PPAR gamma transcription and certain downstream genes involved in lipid lipolysis, transport, and metabolism. The triglyceride and total cholesterol concentrations in the larvae were significantly reduced following exposure to TBPH or TBMEHP. Furthermore, significant increases in the whole ATP content and locomotor activity in the 120 hpf larvae were observed. The overall results suggest that both TBPH and TBMEHP affect methylation of the PPAR gamma promoter, subsequently influencing larvae lipid metabolism via the PPAR gamma signaling pathway and disrupting energy homeostasis. |
| WOS关键词 | POLYBROMINATED DIPHENYL ETHERS ; HALOGENATED FLAME RETARDANTS ; GENE ; TOXICITY ; REVEALS ; DIVERSE |
| 资助项目 | National Natural Science Foundation of China[21737005] ; Strategic Priority Research Program of the Chinese Academy of Sciences[XBD14040103] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2019FBZ03] |
| WOS研究方向 | Engineering ; Environmental Sciences & Ecology |
| 语种 | 英语 |
| 出版者 | AMER CHEMICAL SOC |
| WOS记录号 | WOS:000506723200038 |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/35450]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 通讯作者 | Zhou, Bingsheng |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Zhejiang Univ, Inst Pesticide & Environm Toxicol, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310058, Zhejiang, Peoples R China 3.Wuhan Univ, Inst Adv Studies, Coll Life Sci, Wuhan 430072, Hubei, Peoples R China 4.Minist Ecol & Environm, Nanjing Inst Environm Sci, Nanjing 210042, Jiangsu, Peoples R China 5.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Wei,Hang, Jian,Wu, Shengmin,et al. Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate Affects Lipid Metabolism in Zebrafish Larvae via DNA Methylation Modification[J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY,2020,54(1):355-363. |
| APA | Guo, Wei,Hang, Jian,Wu, Shengmin,Shi, Xiongjie,Wang, Qiangwei,&Zhou, Bingsheng.(2020).Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate Affects Lipid Metabolism in Zebrafish Larvae via DNA Methylation Modification.ENVIRONMENTAL SCIENCE & TECHNOLOGY,54(1),355-363. |
| MLA | Guo, Wei,et al."Bis(2-ethylhexyl)-2,3,4,5-tetrabromophthalate Affects Lipid Metabolism in Zebrafish Larvae via DNA Methylation Modification".ENVIRONMENTAL SCIENCE & TECHNOLOGY 54.1(2020):355-363. |
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来源：水生生物研究所
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