Histopathological examination and transcriptome analyses to assess the acute toxic effects of arsenite exposure on rare minnows (Gobiocypris rarus)
文献类型：期刊论文
| 作者 | Shi, Lixia1,3,4,5; Hu, Xudong3,4; Wang, Nenghan3,4; Liang, Huifang2; Wu, Chenxi3; Cao, Hong1,3 |
| 刊名 | ECOTOXICOLOGY
 |
| 出版日期 | 2020-05-08 |
| 页码 | 12 |
| 关键词 | Acute arsenic toxicity Rare minnow Histopathology Mitochondria Transcriptome |
| ISSN号 | 0963-9292 |
| DOI | 10.1007/s10646-020-02222-3 |
| 通讯作者 | Cao, Hong(regancao@ihb.ac.cn) |
| 英文摘要 | Arsenic is ubiquitously present in the aquatic environment. We investigated the acute toxic effects of arsenite [As(III)] exposure on rare minnows (Gobiocypris rarus) in vivo. The 96-h LC50 value for exposure to As(III) was 13.73 mg/L. As(III) bioaccumulation in different tissues was measured using inductively-coupled plasma mass spectrometry, and the extent of As(III) accumulation was, from greatest to least, liver > intestine > gills > muscle > kidney > testis > brain. Histological examination revealed that in As(III)-treated fish, numerous cellular and tissue alterations were present in the gill, liver, and intestine tissues. Moreover, transmission electron microscopy showed ultrastructural alterations in hepatocytes. We also performed transcriptome analyses to investigate As(III)-induced toxicity response in the liver of As(III)-treated fish; various oxidative-related genes were differentially expressed, and their expression levels were further validated using qPCR. This study is one of the many steps we aim to take on the way to promote the rare minnow to an international standard laboratory animal. |
| WOS关键词 | MITOCHONDRIAL PERMEABILITY TRANSITION ; FRESH-WATER FISH ; DRINKING-WATER ; LIVER-TISSUE ; GILL ; HEALTH ; BIOTRANSFORMATION ; IDENTIFICATION ; METABOLISM ; BIOMARKERS |
| 资助项目 | Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS[LFBC0903] |
| WOS研究方向 | Environmental Sciences & Ecology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000531129300002 |
| 出版者 | SPRINGER |
| 资助机构 | Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS ; Key Lab of Freshwater Biodiversity Conservation Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, CAFS |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/36647]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 通讯作者 | Cao, Hong |
| 作者单位 | 1.CAFS, Key Lab Freshwater Biodivers Conservat, Yangtze River Fisheries Res Inst, Minist Agr & Rural Affairs, Wuhan 430223, Peoples R China 2.Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Hepat Surg Ctr, Wuhan 430030, Peoples R China 3.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat Chinese Acad, Inst Hydrobiol, Wuhan 430072, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 5.Huizhou Univ, Sch Life Sci, Huizhou 516007, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Lixia,Hu, Xudong,Wang, Nenghan,et al. Histopathological examination and transcriptome analyses to assess the acute toxic effects of arsenite exposure on rare minnows (Gobiocypris rarus)[J]. ECOTOXICOLOGY,2020:12. |
| APA | Shi, Lixia,Hu, Xudong,Wang, Nenghan,Liang, Huifang,Wu, Chenxi,&Cao, Hong.(2020).Histopathological examination and transcriptome analyses to assess the acute toxic effects of arsenite exposure on rare minnows (Gobiocypris rarus).ECOTOXICOLOGY,12. |
| MLA | Shi, Lixia,et al."Histopathological examination and transcriptome analyses to assess the acute toxic effects of arsenite exposure on rare minnows (Gobiocypris rarus)".ECOTOXICOLOGY (2020):12. |
入库方式： OAI收割
来源：水生生物研究所
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