中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 水生生物研究所
  3. 中科院水生所知识产出（2009年前）
  4. 水生生物分子与细胞生物学研究中心
  5. 期刊论文
Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9

文献类型：期刊论文

作者Sun, Yonghua6; Zhang, Bo4; Luo, Lingfei3; Shi, De-Li2,5,15; Wang, Han33; Cui, Zongbin6; Huang, Honghui3; Cao, Ying1; Shu, Xiaodong31; Zhang, Wenqing30
刊名GENOME RESEARCH
出版日期2020
卷号30期号:1页码:118-126
ISSN号1088-9051
DOI10.1101/gr.248559.119
通讯作者Liu, Feng(liuf@ioz.ac.cn) ; Zhu, Zuoyan(zyzhu@ihb.ac.cn) ; Meng, Anming(mengam@mail.tsinghua.edu.cn)
英文摘要Genome editing by the well-established CRISPR/Cas9 technology has greatly facilitated our understanding of many biological processes. However, a complete whole-genome knockout for any species or model organism has rarely been achieved. Here, we performed a systematic knockout of all the genes (1333) on Chromosome 1 in zebrafish, successfully mutated 1029 genes, and generated 1039 germline-transmissible alleles corresponding to 636 genes. Meanwhile, by high-throughput bioinformatics analysis, we found that sequence features play pivotal roles in effective gRNA targeting at specific genes of interest, while the success rate of gene targeting positively correlates with GC content of the target sites. Moreover, we found that nearly one-fourth of all mutants are related to human diseases, and several representative CRISPR/Cas9-generated mutants are described here. Furthermore, we tried to identify the underlying mechanisms leading to distinct phenotypes between genetic mutants and antisense morpholino-mediated knockdown embryos. Altogether, this work has generated the first chromosome-wide collection of zebrafish genetic mutants by the CRISPR/Cas9 technology, which will serve as a valuable resource for the community, and our bioinformatics analysis also provides some useful guidance to design gene-specific gRNAs for successful gene editing.
WOS关键词INSERTIONAL MUTAGENESIS ; DRUG DISCOVERY ; WIDE ANALYSIS ; RUNX1 ; CELL ; PROLIFERATION ; MUTATIONS ; PROTEIN ; MODEL ; CAS9
资助项目Ministry of Science and Technology of China[2018YFA0801000] ; Ministry of Science and Technology of China[2018YFA0800200] ; National Natural Science Foundation of China[31830061] ; National Natural Science Foundation of China[31721005] ; Strategic Priority Research Program of the Chinese Academy of Sciences, China
WOS研究方向Biochemistry & Molecular Biology ; Biotechnology & Applied Microbiology ; Genetics & Heredity
语种英语
WOS记录号WOS:000506573000011
出版者COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT
资助机构Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; Ministry of Science and Technology of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China ; Strategic Priority Research Program of the Chinese Academy of Sciences, China
源URL[http://ir.ihb.ac.cn/handle/342005/35481]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者Liu, Feng; Zhu, Zuoyan; Meng, Anming
作者单位1.Tongji Univ, Sch Life Sci & Technol, Shanghai 200092, Peoples R China
2.Guangdong Med Univ, Zhanjiang 524023, Guangdong, Peoples R China
3.Southwest Univ, Sch Life Sci, Chongqing 400715, Peoples R China
4.Peking Univ, Key Lab Cell Proliferat & Differentiat, Minist Educ, Genome Editing Res Ctr,Coll Life Sci, Beijing 100871, Peoples R China
5.Guangdong Med Univ, Affiliated Hosp, Zhanjiang 524001, Peoples R China
6.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Innovat Acad Seed Design, Wuhan 430072, Hubei, Peoples R China
7.Shanghai Jiao Tong Univ, Sino French Res Ctr Life Sci & Genom, Rui Jin Hosp, Sch Med, Shanghai 200025, Peoples R China
8.Fudan Univ, Inst Brain Sci, Shanghai 200433, Peoples R China
9.Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Sichuan, Peoples R China
10.Chinese Acad Sci, Inst Hlth Sci, Shanghai 200025, Peoples R China
推荐引用方式
GB/T 7714		 Sun, Yonghua,Zhang, Bo,Luo, Lingfei,et al. Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9[J]. GENOME RESEARCH,2020,30(1):118-126.
APA Sun, Yonghua.,Zhang, Bo.,Luo, Lingfei.,Shi, De-Li.,Wang, Han.,...&Sun, Jiawei.(2020).Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9.GENOME RESEARCH,30(1),118-126.
MLA Sun, Yonghua,et al."Systematic genome editing of the genes on zebrafish Chromosome 1 by CRISPR/Cas9".GENOME RESEARCH 30.1(2020):118-126.

入库方式： OAI收割

来源：水生生物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。