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Dynamic Phosphoproteome Profiling of Zebrafish Embryonic Fibroblasts during Cold Acclimation

文献类型：期刊论文

作者Yan, Junjun2,3; Long, Yong2; Zhou, Tong2,3; Ren, Jing2,3; Li, Qing2; Song, Guili2; Cui, Zongbin1,2
刊名PROTEOMICS
出版日期2020
卷号20期号:2页码:11
关键词cold acclimation cold exposure phosphoproteome profiling signal transduction zebrafish embryonic fibroblasts
ISSN号1615-9853
DOI10.1002/pmic.201900257
通讯作者Cui, Zongbin(zbcui@ihb.ac.cn)
英文摘要Temperature affects almost all aspects of the fish life. To cope with low temperature, fish have evolved the ability of cold acclimation for survival. However, intracellular signaling events underlying cold acclimation in fish remain largely unknown. Here, the formation of cold acclimation in zebrafish embryonic fibroblasts (ZF4) is monitored and the phosphorylation events during the process are investigated through a large-scale quantitative phosphoproteomic approach. In total, 11 474 phosphorylation sites are identified on 4066 proteins and quantified 5772 phosphosites on 2519 proteins. Serine, threonine, and tyrosine (Ser/Thr/Tyr) phosphorylation accounted for 85.5%, 13.3%, and 1.2% of total phosphosites, respectively. Among all phosphosites, 702 phosphosites on 510 proteins show differential regulation during cold acclimation of ZF4 cells. These phosphosites are divided into six clusters according to their dynamic changes during cold exposure. Kinase-substrate prediction reveals that mitogen-activated protein kinase (MAPK) among the kinase groups is predominantly responsible for phosphorylation of these phosphosites. The differentially regulated phosphoproteins are functionally associated with various cellular processes such as regulation of actin cytoskeleton and MAPK signaling pathway. These data enrich the database of protein phosphorylation sites in zebrafish and provide key clues for the elucidation of intracellular signaling networks during cold acclimation of fish.
WOS关键词DEPENDENT PROTEIN-KINASE ; IN-VIVO ; C-JUN ; MEDIATES COLD ; HIGH-ACCURACY ; HOG PATHWAY ; PHOSPHORYLATION ; TEMPERATURE ; REVEALS ; ACTIVATION
资助项目Science Fund for Creative Research Group of National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31872554] ; National Natural Science Foundation of China[31572610] ; National Natural Science Foundation of China[31772836] ; National Natural Science Foundation of China[31401052]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000506064600001
出版者WILEY
资助机构Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; Science Fund for Creative Research Group of National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/35513]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者Cui, Zongbin
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Yan, Junjun,Long, Yong,Zhou, Tong,et al. Dynamic Phosphoproteome Profiling of Zebrafish Embryonic Fibroblasts during Cold Acclimation[J]. PROTEOMICS,2020,20(2):11.
APA Yan, Junjun.,Long, Yong.,Zhou, Tong.,Ren, Jing.,Li, Qing.,...&Cui, Zongbin.(2020).Dynamic Phosphoproteome Profiling of Zebrafish Embryonic Fibroblasts during Cold Acclimation.PROTEOMICS,20(2),11.
MLA Yan, Junjun,et al."Dynamic Phosphoproteome Profiling of Zebrafish Embryonic Fibroblasts during Cold Acclimation".PROTEOMICS 20.2(2020):11.

入库方式： OAI收割

来源：水生生物研究所

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