Proteomic identification and expression of oral apparatus constituents in cell regeneration of giant ciliate Stentor coeruleus (strain WHEL)
文献类型：期刊论文
| 作者 | Wei, Wei1,4; Jiang, Chuanqi1; Yang, Wentao1,4; Miao, Wei1,2,3; Xiong, Jie1 |
| 刊名 | GENE
 |
| 出版日期 | 2020-06-15 |
| 卷号 | 743页码:7 |
| 关键词 | Protozoan Oral Apparatus Associated Genes Proteome Gene Expression Single-cell RNA-sequencing |
| ISSN号 | 0378-1119 |
| DOI | 10.1016/j.gene.2020.144624 |
| 通讯作者 | Xiong, Jie(xiongjie@ihb.ac.cn) |
| 英文摘要 | The giant ciliate Stentor coeruleus (S. coeruleus) is a suitable model organism for studying morphogenesis and regeneration at the single-cell level. It contains a prominent structure on the anterior end of the cell, known as the oral apparatus (OA). OA can be induced to shed by urea treatment and then new OA regenerates via a series of defined morphological events and the cell resumes normal feeding activity. We identified OA constituents in S. coeruleus by mass spectrometry. A total of 882 OA-associated proteins were identified; the homologs of 181 of these are known OA constituents in other organisms. The expression pattern of OA-associated genes during regeneration was investigated using single-cell transcriptome sequencing. The expression of most OA-associated genes was high during regeneration, indicating their stable expression after OA shedding. We also identified OA-associated differentially expressed genes that may be involved in regulating OA reconstruction. In summary, this study gives preliminary insight into the molecular basis of OA in S. coeruleus. |
| WOS关键词 | MOLECULAR CHAPERONES ; PROTEIN ; TETRAHYMENA ; FLAGELLA ; HEAT-SHOCK-PROTEIN-40 ; MICROTUBULES ; CALMODULIN ; TOPOLOGY ; GENOME ; DOMAIN |
| 资助项目 | National Natural Science Foundation of China[31525021] ; National Natural Science Foundation of China[31672281] ; National Natural Science Foundation of China[31872221] ; Youth Innovation Promotion Association of the Chinese Academy of Sciences |
| WOS研究方向 | Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000529796500016 |
| 出版者 | ELSEVIER |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences ; Youth Innovation Promotion Association of the Chinese Academy of Sciences |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/36441]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 通讯作者 | Xiong, Jie |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquat Biodivers & Conservat, Wuhan 430072, Peoples R China 2.CAS Ctr Excellence Anim Evolut & Genet, Kunming 650223, Yunnan, Peoples R China 3.State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wei, Wei,Jiang, Chuanqi,Yang, Wentao,et al. Proteomic identification and expression of oral apparatus constituents in cell regeneration of giant ciliate Stentor coeruleus (strain WHEL)[J]. GENE,2020,743:7. |
| APA | Wei, Wei,Jiang, Chuanqi,Yang, Wentao,Miao, Wei,&Xiong, Jie.(2020).Proteomic identification and expression of oral apparatus constituents in cell regeneration of giant ciliate Stentor coeruleus (strain WHEL).GENE,743,7. |
| MLA | Wei, Wei,et al."Proteomic identification and expression of oral apparatus constituents in cell regeneration of giant ciliate Stentor coeruleus (strain WHEL)".GENE 743(2020):7. |
入库方式： OAI收割
来源：水生生物研究所
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